Rabbit anti lmp1

Dimethyl sulfoxide (DMSO) was obtained from Sigma–Aldrich (Cat: D2650, St. Louis, MO, USA). Octyl-α-ketoglutarate (octyl-α-KG) and octyl-α-hydroxyglutarate (octyl-2-HG) were from Cayman (Cat: 11970 and 16366, Ann Arbor, MI, USA). AGI-6780 and MG132 were purchased from MedChemExpress (Cat: HY-15734 and HY-13259, Monmouth Junction, NJ, USA).
The following antibodies were used: mouse anti-IDH2 (Cat: ab55271, Abcam, Cambridge, MA, USA), mouse anti-β-actin (Cat: A5441, Sigma), rabbit anti-LMP1 (Cat: ab78113, Abcam), rabbit anti-IDH1 (Cat: ab172964, Abcam), and mouse anti-c-Myc (Cat: sc-40, Santa Cruz, Dallas, TX, USA).
The expression vectors for IDH2 and LMP1 have been described previously [7 (link),14 (link)].

Human TNFα was purchased from Peprotech (Cat: 300-01A, Rocky Hill, NJ, USA). Dimethyl fumarate (DMF), DMSO, and z-VAD-fmk were obtained from Sigma-Aldrich (Cat: V900731, D2650, and C2105, St. Louis, MO, USA). Octyl-α-ketoglutarate (octyl-α-KG) was from Cayman (Cat: 11970, Ann Arbor, MI, USA). Trichostatin A (TSA) and nicotinamide (NAM) were purchased from MCE (Cat: HY-15144 and HY-B0150, Monmouth Juncton, NJ, USA). Smac mimetic and 5-aza-dC were from Selleck (Cat: S7010 and S1200, Houston, TX, USA). TNF-α (T, 100 ng/ml), Smac mimetic (S, 5 μM), and z-VAD-fmk (Z, 20 μM) were used as necroptosis inducers (T/S/Z).
Antibodies used were mouse anti-acetylation (Cat: 66289-1-Ig, Proteintech, Rosemont, IL, USA), mouse anti-β-actin (Cat: A5441, Sigma), rabbit anti-FH (Cat: 11375-1-AP, Proteintech), rabbit anti-LMP1 (Cat: ab78113, Abcam, Cambridge, MA, USA), rabbit anti-MLKL (Cat: M6697, Sigma), rabbit anti-phosphorylated MLKL (Cat: ab187091, Abcam), rabbit anti-RIP3 (Cat: ab56164, Abcam), mouse anti-TET1 (Cat: 61444, Active Motif, Carlsbad, CA, USA).