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4 protocols using hexanoic acid

1

Peanut Seed Pretreatment and Fatty Acid Extraction

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Dehulled peanut seeds were obtained from the Zhengzhou Pengxun Seed Industry Co., Ltd. (Zhengzhou, China), called Yuanza-6 (YZ-6). Peanut seeds were roasted at 50 °C ovens to remove the red coat and stored at 4 °C. Viscozyme® L was purchased from Novozymes Co., Ltd. (Shanghai, China). Several fatty acids were acquired from Macklin Co., Ltd (Shanghai, China), including valeric acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, and decanoic acid.
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2

Chromatin Regulation Assay in Drosophila

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Sucrose (#S0389), NaCl (#S3014), CaCl2 (#C5670), MgCl2 (#M4880), KCl (#P9541), NaH2PO4 (#S3139), NaHCO3 (#S5761), and HEPES (#54457) were from Sigma-Aldrich. Hexanoic acid (Macklin, #H810882) for PER assay was purchased from Macklin. EED226 (5 μM, Selleck, #S8496), Chaetocin (5 μM, Selleck #S8068), and A-395 (10 μM, Sigma-Aldrich, #SML1923) were added to the standard medium. Flies were kept on these foods for 5 days before the assay (change fresh medium every 2 days).
The following antibodies were used: The antibodies against Histone H3 (di methyl K9) (#ab1220), acetyl histone-h3-k27 (#ab4729), Histone H3 (trimethyl K27) (#ab6002, #PTM-5002, #PTM-647RM), Histone H3 (trimethyl K9) (#ab8898), and Histone H3 antibody (#ab1791) were purchased from Abcam and Jingjie PTM BioLab. The secondary antibodies against rabbit (Alexa Fluor 633, #a21071, HRP-linked Antibody, #111-035-003) and mouse (Alexa Fluor 488, #a11001) were purchased from Jackson ImmunoResearch and Invitrogen. Fluoroshield with DAPI (#F6057) was purchased from Sigma-Aldrich.
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3

Chromatin Regulation Assay in Drosophila

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Sucrose (#S0389), NaCl (#S3014), CaCl2 (#C5670), MgCl2 (#M4880), KCl (#P9541), NaH2PO4 (#S3139), NaHCO3 (#S5761), and HEPES (#54457) were from Sigma-Aldrich. Hexanoic acid (Macklin, #H810882) for PER assay was purchased from Macklin. EED226 (5 μM, Selleck, #S8496), Chaetocin (5 μM, Selleck #S8068), and A-395 (10 μM, Sigma-Aldrich, #SML1923) were added to the standard medium. Flies were kept on these foods for 5 days before the assay (change fresh medium every 2 days).
The following antibodies were used: The antibodies against Histone H3 (di methyl K9) (#ab1220), acetyl histone-h3-k27 (#ab4729), Histone H3 (trimethyl K27) (#ab6002, #PTM-5002, #PTM-647RM), Histone H3 (trimethyl K9) (#ab8898), and Histone H3 antibody (#ab1791) were purchased from Abcam and Jingjie PTM BioLab. The secondary antibodies against rabbit (Alexa Fluor 633, #a21071, HRP-linked Antibody, #111-035-003) and mouse (Alexa Fluor 488, #a11001) were purchased from Jackson ImmunoResearch and Invitrogen. Fluoroshield with DAPI (#F6057) was purchased from Sigma-Aldrich.
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4

Volatile Compound Identification Standards

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Standards were purchased for volatile compound identification. 2-Pentylfuran and trans-3-octen-2-one were provided by Sigma Aldrich (St. Louis, MO, USA). Other compounds, including hexanal, benzaldehyde, octanal, nonanal, heptanal, 3-methylbutanal, propanol, pentanol, hexanol, ethanol, 1-octen-3-ol, 3-methyl-1-butanol, 2-methylpropanol, phenethyl alcohol, acetoin, 6-methyl-5-hepten-2-one, 2,3-butanedione, acetic acid, octanoic acid, pentanoic acid, hexanoic acid, γ-undecalactone, ethyl acetate, hexyl acetate, were provided by Macklin (Shanghai, China).
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