Sodium alginate with a medium viscosity and high mannuronic acid without inulin were purchased from Hi-media (Mumbai). They were prepared in distilled water and autoclaved at 121 °C for 15 min. The low-molecular-weight chitosan (deacetylated chitin, Hi-Media, Laboratories Mumbai), was prepared in distilled water and pure hydrochloric acid (Loba Chemie, Pvt Ltd.—Mumbai, India). Xanthan gum, gum acacia, and carrageenan were purchased from Loba Chemie, Pvt Ltd.—Mumbai, India. Starch, sodium caseinate, pepsin, trypsin, calcium chloride, sodium chloride, sodium bicarbonate, trisodium citrate, sodium hydroxide, potassium chloride, phosphate buffer saline (pH 7.2), and hydrochloric acid were purchased from Hi-media Laboratories (Mumbai, India). Glacial acetic acid with molar mass of 60.05 g mol−1 was bought from Merck (Darmstadt, Germany). Pancreatin, peptone, and bile salt were obtained from Loba Chemie, Pvt Ltd. (Mumbai, India). The De Man Rogosa and Sharpes (MRS) broth and MRS agar used in this work were purchased from Hi-media Laboratories (Mumbai). Indicator microorganisms used for the antimicrobial activity; i.e., Escherichia coli (MTCC No-432), Staphylococcus aureus (MTCC No-96), and Bacillus cereus (MTCC No-430), were procured from the Institute of Microbial Technology (IMTECH; Chandigarh, India).
Sodium bicarbonate
Manufactured by HiMedia
Sourced in India, United States
Sodium bicarbonate is a chemical compound with the formula NaHCO3. It is a white, crystalline solid that is commonly used as a leavening agent in baking and as an antacid for the treatment of acid reflux and indigestion.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by HiMedia (6 mentions)
Sodium hydroxide (naoh), by HiMedia (5 mentions)
Sodium carbonate, by HiMedia (5 mentions)
Calcium chloride, by HiMedia (2 mentions)
Potassium chloride (kcl), by HiMedia (2 mentions)
Trichloroacetic acid, by HiMedia (2 mentions)
Dmem medium, by HiMedia (2 mentions)
Rhodamine 123, by Merck Group (2 mentions)
Sodium sulfate, by HiMedia (2 mentions)
Formaldehyde, by Merck Group (2 mentions)
Disodium hydrogen phosphate, by HiMedia (2 mentions)
Sodium dihydrogen phosphate, by HiMedia (2 mentions)
Glucose, by HiMedia (2 mentions)
Sodium acetate, by HiMedia (2 mentions)
Magnesium chloride, by HiMedia (2 mentions)
Sodium azide, by HiMedia (2 mentions)
Sodium caseinate, by HiMedia (1 mentions)
Trisodium citrate, by HiMedia (1 mentions)
Peptone, by Loba Chemie (1 mentions)
Hydrochloric acid (hcl), by HiMedia (1 mentions)
20 protocols using sodium bicarbonate
1
Natural Antimicrobial Polymer Formulations
2
Antioxidant Analysis of Broccoli
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Different chemical reagents used for analysis viz. ascorbic acid, calcium chloride, quercetin, aluminium chloride, sodium nitrite, sodium hydroxide pellets, gallic acid, folin-ciocalteu phenol reagent, anhydrous sodium carbonate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium bicarbonate, methanol, tri-chloro acetic acid, and acetone were of analytical grade and purchased from Sigma-Aldrich (St. Louis, USA) and Himedia (HiMedia Laboratories, Mumbai, India). Broccolis (cultivar: Pusa KTS-1) utilized in the study were obtained from the Farm section of ICAR-Central Institute of Agricultural Engineering in Bhopal, India (23.3156° N, 77.4037° E). The broccolis were harvested 125 days after sowing of seeds and 90 days of transplanting. Mass of individual heads were in the range of 350–450 g.
3
Transient Transfection of HEK 293T Cells
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HEK 293T cells (obtained from ATCC CRL-3216) were cultured in T25 flasks (Nunc™) using DMEM high glucose (Thermo Fisher Scientific, MA) supplemented with 10% foetal bovine serum (Invitrogen, Carlsbad, CA, USA), 2 g/l sodium bicarbonate (Himedia, India) and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific, MA, USA). Cells were incubated in a humidified chamber at a temperature of 37°C and 5% CO2 (Thermo Fisher Scientific). Cells were seeded in six-well plates with a density of 5×105 cells per well and transfection was performed using Lipofectamine 2000™ (Invitrogen), according to the manufacturer's protocol. Briefly, 1 μg of target gene containing plasmid and 2 μg of miRNA-encoding plasmid were mixed with 2.5 μl of Lipofectamine in OptiMEM (Invitrogen) and incubated for 30 min at room temperature for complex formation. The mixture was added to the cells, incubated at 37°C for 4 h and complemented with complete DMEM followed by further incubation at 37°C, 5% CO2 for 48 h.
4
Cell Culture Reagents and Cell Lines
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For cell culture, DMEM medium, Sodium bicarbonate, antibiotics, and others were obtained from standard manufactures (Invitrogen, Sigma and Himedia). Various antibodies were purchased from Cell Signaling Technology, Beverly, MA, USA and Santa Cruz Biotechnology, USA. IL-6R siRNA was purchased from Santa Cruz Biotechnology, USA. 5-Azacytidin (5-Aza) and Diacerein (Dia) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Other chemicals were purchased from Sigma-Aldrich, St. Louis, MO, USA and Himedia India. MDA-MB-468 and MDA-MB-231 cells were purchased from National Center for Cell Science, Pune, India. HUVEC cells were purchased from Himedia India.
5
Silk Fibroin-Based Biomaterials for Cancer Therapeutics
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Silk cocoons
of B. mori (silkworm) were procured
from Central Silk Board, Dehradun, Uttarakhand, India. Poly(vinyl
alcohol) (PVA) (HiMedia, cat. no. GRM6170), sodium carbonate (HiMedia,
cat.no. GRM851), sodium bicarbonate (HiMedia, cat. no. MB045), lithium
bromide (SRL, cat. no. 31665), dialysis membrane (MWCO 12 kDa, HiMedia,
cat. no. LA401), ethanol (CSS, cat.no. 1170), glutaraldehyde (25%,
for synthesis, LOBA CHEMIE Pvt. Ltd., cat no. 03965), and formic acid,
98% AR (LOBA CHEMIE Pvt. Ltd., cat no. 00153) were purchased and used
as received. MCF-7 cell line (breast cancer cell line) was obtained
from NCCS, Pune, and cultured in Dulbecco’s modified eagle
medium (DMEM) (HiMedia, cat.no. AT007) containing 10% fetal bovine
serum (FBS, Gibco, cat. no. 10270–106) and 1% penicillin–streptomycin
(Gibco, cat. no. 10378-016). For cell culture experiments, Dulbecco’s
phosphate-buffered saline (DPBS, Ca2+- and Mg2+-free, Sigma-Aldrich, cat.no. D5652), trypsin-ethylenediaminetetraacetic
acid (EDTA) (0.25%, Sigma-Aldrich, cat. no. 25200-070), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide, Amresco, cat.no. 0793), Hoechst 33342 (Sigma-Aldrich, cat.
no. 14533), and Rhodamine 123 (Sigma-Aldrich, cat. no. R8004) were
used.
of B. mori (silkworm) were procured
from Central Silk Board, Dehradun, Uttarakhand, India. Poly(vinyl
alcohol) (PVA) (HiMedia, cat. no. GRM6170), sodium carbonate (HiMedia,
cat.no. GRM851), sodium bicarbonate (HiMedia, cat. no. MB045), lithium
bromide (SRL, cat. no. 31665), dialysis membrane (MWCO 12 kDa, HiMedia,
cat. no. LA401), ethanol (CSS, cat.no. 1170), glutaraldehyde (25%,
for synthesis, LOBA CHEMIE Pvt. Ltd., cat no. 03965), and formic acid,
98% AR (LOBA CHEMIE Pvt. Ltd., cat no. 00153) were purchased and used
as received. MCF-7 cell line (breast cancer cell line) was obtained
from NCCS, Pune, and cultured in Dulbecco’s modified eagle
medium (DMEM) (HiMedia, cat.no. AT007) containing 10% fetal bovine
serum (FBS, Gibco, cat. no. 10270–106) and 1% penicillin–streptomycin
(Gibco, cat. no. 10378-016). For cell culture experiments, Dulbecco’s
phosphate-buffered saline (DPBS, Ca2+- and Mg2+-free, Sigma-Aldrich, cat.no. D5652), trypsin-ethylenediaminetetraacetic
acid (EDTA) (0.25%, Sigma-Aldrich, cat. no. 25200-070), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide, Amresco, cat.no. 0793), Hoechst 33342 (Sigma-Aldrich, cat.
no. 14533), and Rhodamine 123 (Sigma-Aldrich, cat. no. R8004) were
used.
6
Microbial and Nematode Assay Protocols
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All the strains used in this study, Chromobacterium violaceum (ATCC 12472) and P. aeruginosa PAO1 (ATCC 15692), were maintained in glycerol (80%) and stored at −80°C. For every new experiment, fresh stocks were subcultured. The wild-type strain of Caenorhabditis elegans (Bristol N2) was used for the in vivo experiments. Furanone C-30 (CAS No.: 247167-54-0, product No.: 53796) was purchased from Merck, India. Analytical grade chemicals and solvents were used for the synthesis of activated furans. Furfuraldehyde (CAS No.: 98–01-1, Item No.: 0106146), Meldrum’s acid (CAS No.: 2033-24-1, Item No.: 0113207), 1,3-dimethyl barbituric acid (CAS No.: 769–42-6 Item No.: 0104393), and dichloromethane (CAS No.: 75–09-2, Item No.: 0113271) were purchased from Spectrochem, India. Sodium bisulphite (CAS No.: 7631-90-5, MB358), sodium bicarbonate (CAS No.: 144–55-8, PCT1535), and sodium sulfate (CAS No.: 7757-82-6, GRM6425) were purchased from HiMedia, India.
7
Extraction and Analysis of Bioactive Compounds
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Fresh fruits of M. charantia and A. esculentus were obtained from a vegetable garden along the northern bypass road at 0°34’53.0"S, 30°39’30.0"E, (Mbarara, Uganda), ethanol absolute, Molecular weight (Mwt) = 46.07 (VWR, chemicals, France), Sodium citrate 99% assay, Mwt = 294.10 (UNILAB Kenya Limited, Nairobi, Kenya), industrial-grade Sodium bicarbonate, Mwt = 84.01 (HiMedia Laboratories, Mumbai, India), Magnesium carbonate, Mwt = 84.31 (Thomas Baker Chemicals Limited, Mumbai, India), Lactose, Mwt = 342.30 (BDH laboratory suppliers, Kampala, Uganda), Talc (IDA, Merch, Eurolab, Belgium), Folin-Ciocalteu reagent (Labo Chemie Pvt Limited, Mumbai, India), HPLC grade gallic acid, Mwt = 170.12 (Labo Chemie Pvt Limited, Mumbai, India), Quercetin, Mwt = 302.24 (Sigma-Aldrich, St. Louis, USA). Also, HPLC grade; methanol, Mwt = 32.04, acetonitrile, Mwt = 41.05, and trifluoroacetic acid, Mwt = 114.02 were obtained from (Labo Chemie Pvt Limited, Mumbai, India), distilled water was in-house prepared. Other materials were used, including a shaking water bath (OLS 200, Grants, UK) and a rotary evaporator (RV 10D S99, IKA, German).
8
Nanoparticle Formulation for Anticancer Drug Delivery
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PLGA (50:50 7 kDa–17 kDa, acid terminated), polyethylenimine (25 kDa, branched), epirubicin and paclitaxel were purchased from Sigma Aldrich (Darmstadt, Germany). Acetone was purchased from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). PF68 surfactant, Alamar blue, PBS buffer and sodium bicarbonate were procured from HiMedia (Mumbai, India). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) were obtained from Gibco Life Technologies (Carlsbad, CA, USA). In-house distilled water was used during the studies.
9
Metallohydrogel Synthesis and Characterization
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2,4-Di-tert-butylphenol was purchased from Sigma-Aldrich. Manganese acetate, aspartic acid and indomethacin were obtained from alfa SRL. Gemcitabine was bought from BLD pharm. Commercially available forms of formaldehyde, ethanol, and methanol was employed without additional purification. Milli Q water was used for the formation of metallohydrogel. Other common solvents of the analytical reagent grade were utilised. DMEM medium, sodium bicarbonate, antibiotic solution and FBS, propidium iodide and calcein AM were purchased from Himedia, Sigma-Aldrich, and invitrogen.
10
Comprehensive Analytical Protocol for Pharmaceutical Compounds
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Sodium phosphate dibasic dehydrate (sigma Aldrich, Germany), Sodium phosphate monobasic dihydrate (Acros organics, United States), Distilled deionized water, Sodium chloride (HiMedia Laboratories, India), Sodium Diclofenac (Amoli Organics Pvt., India), Formaldehyde (Merck, Germany), Anhydride acetic acid (Acros organics, USA), Picric acid (BDH laboratory, England), Hydrochloric acid (Chemlab, Belgium), Chloroform (Eurolab, UK), Sodium bicarbonate (Himedia, India), Sodium hydroxide (Himedia, India), 3,5 di-nitro Binzoic acid (Merck, Germany), Vanillin (Merck, Germany), Aluminum chloride (Merck, Germany), Ferric chloride (Merck, Germany), Bismuth nitrate (Merck, Germany), Potassium iodide (Rectapur, prolab, CE), Ethanol (Schalau SL, Spain) and Sulfuric acid (Surechem product LTD, England). Apparatus used in this study were Electronic balance (Sartoruis AG, Germany), Ultrapure TM aqua purification system (Lotun Co., Ltd., Taipei, Taiwan), UV-1800 spectrophotometer (Shimadzu, Japan), Water bath, Rotary evaporator (Heidolph Instruments, Germany) and TSE Analgesia Meter Tail Flick (tinateb, USA).
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