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Ab181136

Manufactured by Abcam
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Ab181136 is an antibody reagent produced by Abcam. It is a monoclonal antibody that recognizes a specific target molecule. The core function of this product is to serve as a tool for detecting and analyzing the target antigen in various research and diagnostic applications.

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Lab products found in correlation

Ab191080, by Abcam (2 mentions) Ab202975, by Abcam (2 mentions) Ab138490, by Abcam (2 mentions) Ab195121, by Abcam (2 mentions) Ab192985, by Abcam (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Vinculin, by Merck Group (2 mentions) Ab230445, by Abcam (2 mentions) Ab15580, by Abcam (2 mentions) Ab1791, by Abcam (2 mentions) Sc 7392, by Santa Cruz Biotechnology (2 mentions) Baf a1 s1413, by Selleck Chemicals (2 mentions) Gtx121246, by GeneTex (2 mentions) Sc 17775, by Santa Cruz Biotechnology (2 mentions) Sc 373822, by Santa Cruz Biotechnology (2 mentions) Vegfc, by ABclonal (2 mentions) F3165 1mg, by Merck Group (2 mentions) Myc tag, by Proteintech (2 mentions) Ha tag, by Proteintech (2 mentions) Gsk126, by Selleck Chemicals (2 mentions)

3 protocols using ab181136

1

Wnt Signaling Pathway Modulation

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pCDNA-V5-FZD5 was a kind gift from Dr. Hans Clevers. TCF4-DN in pLX303 was a gift from Dr. William Hahn (Addgene plasmid # 42592). Two distinct DsiRNA-DDB2, two DsiRNA-EZH2 and non-targeting DsiRNA-Control were purchased from IDT DNA pre-designed library. The sequences are listed in supplementary table S1. ON-TARGET plus PAF siRNA smartpool and non-targeting siRNA smartpool were purchased from Dharmacon. Antibodies used in this study include DDB2 (Western Blotting: 5416, Cell Signaling; Chromatin Immunoprecipitation: sc-25368, Santa Cruz; co-Immunoprecipitation: ab181136, Abcam), EZH2 (Western Blotting: 5246, Cell Signaling; Chromatin Immunoprecipitation: 39875, Active Motif), β-catenin (8480, Cell Signaling), RNF43 (orb140091, Biorbyt), EED (sc-28701, Santa Cruz), SUZ12 (3737, Cell Signaling), TCF4 (2569, Cell Signaling), V5 tag (R960-25, Invitrogen), T7 tag (69522, EMD Millipore), α-Tubulin (T9026, Sigma-Aldrich) and LRP5/6 (bs-2905R, BIOSS). HRP-conjugated secondary antibodies were purchased from BioRad.
Huang S., Fantini D., Merrill B.J., Bagchi S., Guzman G, & Raychaudhuri P. (2017). DDB2 is a Novel Regulator of Wnt-Signaling in Colon Cancer. Cancer research, 77(23), 6562-6575.
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2

Comprehensive Antibody and Inhibitor Panel

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Primary antibodies used in this study are as follows: UTX (33510S, Cell Signaling Technology; GTX121246, GeneTex), COP1 (A300-894A, Bethyl), CUL1 (SC-17775, Santa Cruz Biotechnology), CUL2 (A5308, Abclonal), CUL3 (2759, Cell Signaling Technology), CUL4A (AB60215a, Abgent), CUL4B (A6198, Abclonal), CUL5 (SC-373822, Santa Cruz Biotechnology), EMP1 (ab230445 and ab202975, Abcam), AUTS2 (25,001–1-AP, Proteintech), Ki67 (ab15580, Abcam), EZH2 (ab191080, Abcam), VEGFC (A2556, Abclonal), Caspase-9 (A0281, Abclonal), Bcl2 (15,071, Cell Signaling Technology), DDB2 (ab181136, Abcam), DCAF7 (ab138490, Abcam), DCAF13 (ab195121, Abcam), H3K27me3 (ab192985, Abcam; A2363, Abclonal), H3 (ab1791, Abcam), P27 (610,241, BD), HA-Tag (51,064–2-AP, Proteintech; SC-7392, Santa Cruz Biotechnology), Flag-Tag (F7425-0.2MG, Sigma; F3165-1MG, Sigma), GFP (66,002–1-Ig, Proteintech), Myc-Tag (16,286–1-AP, Proteintech), α-tubulin (SC-23948, Santa Cruz Biotechnology), and Vinculin (V4505, Sigma). The chemical inhibitors MG132 (S2619), MG101 (S7386), MLN4924 (S7109), Baf-A1 (S1413), and GSK126 (S7061) were purchased from Selleck.
Luo D., Chen M., Li Q., Wang K., Wang K., Li J., Fu G., Shan Z., Liu Q., Yang Y., Liang L., Ma Y., Qin Y., Qin J., Gao D, & Li X. (2023). CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. Experimental Hematology & Oncology, 12, 77.
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3

Comprehensive Antibody and Inhibitor Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used in this study are as follows: UTX (33510S, Cell Signaling Technology; GTX121246, GeneTex), COP1 (A300-894A, Bethyl), CUL1 (SC-17775, Santa Cruz Biotechnology), CUL2 (A5308, Abclonal), CUL3 (2759, Cell Signaling Technology), CUL4A (AB60215a, Abgent), CUL4B (A6198, Abclonal), CUL5 (SC-373822, Santa Cruz Biotechnology), EMP1 (ab230445 and ab202975, Abcam), AUTS2 (25,001–1-AP, Proteintech), Ki67 (ab15580, Abcam), EZH2 (ab191080, Abcam), VEGFC (A2556, Abclonal), Caspase-9 (A0281, Abclonal), Bcl2 (15,071, Cell Signaling Technology), DDB2 (ab181136, Abcam), DCAF7 (ab138490, Abcam), DCAF13 (ab195121, Abcam), H3K27me3 (ab192985, Abcam; A2363, Abclonal), H3 (ab1791, Abcam), P27 (610,241, BD), HA-Tag (51,064–2-AP, Proteintech; SC-7392, Santa Cruz Biotechnology), Flag-Tag (F7425-0.2MG, Sigma; F3165-1MG, Sigma), GFP (66,002–1-Ig, Proteintech), Myc-Tag (16,286–1-AP, Proteintech), α-tubulin (SC-23948, Santa Cruz Biotechnology), and Vinculin (V4505, Sigma). The chemical inhibitors MG132 (S2619), MG101 (S7386), MLN4924 (S7109), Baf-A1 (S1413), and GSK126 (S7061) were purchased from Selleck.
Luo D., Chen M., Li Q., Wang K., Wang K., Li J., Fu G., Shan Z., Liu Q., Yang Y., Liang L., Ma Y., Qin Y., Qin J., Gao D, & Li X. (2023). CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. Experimental Hematology & Oncology, 12, 77.
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