Drosophila were grown on corn flour and agar media with brewer’s yeast. All strains were grown at 25 °C. pgcGFP, pgcΔ and pgcΔFRT fly lines were generated for this study. liprinγH1 and bamΔ86 flies were acquired from the Treisman Lab (Astigarraga et al., 2010 ) and McKearin Lab (McKearin and Ohlstein, 1995 (link)) respectively. nos-Gal4::VP16, UASCycB (Mathieu et al., 2013 (link)) and sco/CyO; MKRS/TM6 was acquired from the Lehmann lab. UASpCycT-nos3′UTR, UASpcdk9-nos3′UTR and Genomic pgc+ transgene was given to us by the Nakamura Lab (Hanyu-Nakamura et al., 2008 (link)). CycB2, bamGFP, hsFLP, 42DFRT-GFP, CycA[c03456], CycA[03946] and CycA[c8LR1] are available at Bloomington Drosophila Stock Center, Bloomington, IN (Wang and Lin, 2005 (link)). CycTRNAi (v103387) and cdk9RNAi (v103569) lines were acquired from VDRC stock center.
Bamgfp
Manufactured by Bloomington Drosophila Stock Center
Sourced in United States
The BamGFP is a genetic construct that expresses green fluorescent protein (GFP) under the control of the Bam promoter in Drosophila. It is a tool used to visualize the expression of the Bam gene, which is involved in the regulation of germline stem cell differentiation in the Drosophila ovary.
Automatically generated - may contain errors
Lab products found in correlation
Cyca c8lr1, by Bloomington Drosophila Stock Center (2 mentions)
Hsflp, by Bloomington Drosophila Stock Center (2 mentions)
Cycb2, by Bloomington Drosophila Stock Center (2 mentions)
42dfrt gfp, by Bloomington Drosophila Stock Center (2 mentions)
Cyca c03456, by Bloomington Drosophila Stock Center (2 mentions)
Cyca 03946, by Bloomington Drosophila Stock Center (2 mentions)
Uas egfrdn, by Bloomington Drosophila Stock Center (1 mentions)
Nub gal4, by Bloomington Drosophila Stock Center (1 mentions)
Ap gal4, by Bloomington Drosophila Stock Center (1 mentions)
Nos gal4 vp16, by Bloomington Drosophila Stock Center (1 mentions)
Y1w67c23, by Bloomington Drosophila Stock Center (1 mentions)
Sqh pkn rbd g58a egfp, by Bloomington Drosophila Stock Center (1 mentions)
4 protocols using bamgfp
1
Drosophila Germline Development Protocols
2
Drosophila Germline Development Protocols
3
Drosophila Mutant Strain Catalog
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y w was used as a wild-type strain. The following mutants and transgenic lines were used: nos-gal4VP16[14] (link) from R. Lehmann (New York University, New York, NY, USA); nub-gal4 and ap-gal4[15] (link) from T. Hayashi (NIG, Mishima, Japan); UAS-H2B-ECFP[16] (link) from S. Kondo (NIG, Mishima, Japan); UAS-EgfrDN[17] (link) from M. Freeman (MRC Laboratory of Molecular Biology, Cambridge, UK); UAS-pav-GFP[18] (link) from Y. M. Yamashita (University of Michigan, Ann Arbor, MI, USA); bam-GFP[19] (link) from D. McKearin (HHMI, Chevy Chase, MD, USA); and argosdelta7[20] (link) from the Bloomington Stock Center (Indiana University, Bloomington, IN, USA). goe5–11 and goe331 alleles were generated by imprecise excision of a P element, EY01697, inserted in the 5′UTR of goe.
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y w was used as a wild-type strain. The following mutants and transgenic lines were used: nos-gal4VP16[14] (link) from R. Lehmann (New York University, New York, NY, USA); nub-gal4 and ap-gal4[15] (link) from T. Hayashi (NIG, Mishima, Japan); UAS-H2B-ECFP[16] (link) from S. Kondo (NIG, Mishima, Japan); UAS-EgfrDN[17] (link) from M. Freeman (MRC Laboratory of Molecular Biology, Cambridge, UK); UAS-pav-GFP[18] (link) from Y. M. Yamashita (University of Michigan, Ann Arbor, MI, USA); bam-GFP[19] (link) from D. McKearin (HHMI, Chevy Chase, MD, USA); and argosdelta7[20] (link) from the Bloomington Stock Center (Indiana University, Bloomington, IN, USA). goe5–11 and goe331 alleles were generated by imprecise excision of a P element, EY01697, inserted in the 5′UTR of goe.
4
Genetic Dissection of Planar Cell Polarity Pathways
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The following fly stocks were used in the study: c587-Gal4, trafficJam (tj)-Gal4, dWnt4C1/CyO (6651), bamGFP, arrow RNAi (31313), Rho1 RNAi (Bloomington 32383), UASRhoDN (Bloomington 7327), Rac1 RNAi (Bloomington 28985 and 34910), Mtl RNAi (Bloomington 51932), Rac2 RNAi (v28926), cdc42 RNAi (Bloomington 35756 and 37477), UAScdc42DN (Bloomington 6288), dDAAM1 RNAi (Bloomington 39058, V24885), dsh RNAi (v101525), frizzled RNAi (Bloomington 34321), van gogh RNAi (Bloomington v7376), daschous RNAi (Bloomington 28008), fat RNAi (Bloomington 29566), y1 w*; Rho172F /CyO (Bloomington 7326), y1 w67c23 ; P{EPgy2}Rac1EY05848 /TM6B , Tb1 (Bloomington 15461), y[1] w[*]; Rac1[J10] Rac2[Delta] P{w[+mW.hs] = FRT(w[hs])}2A Mtl[Delta]/TM6B, Tb[1] (Bloomington 6679), y1 w*Cdc423 /FM6 (Bloomington 7337), y1 w*Cdc422P{neoFRT}19A (Bloomington 9105), y1 w*Cdc425P{neoFRT}19A (Bloomington 52237), y1 Mi{MIC}DAAMMI04569 w1118 /FM7h (Bloomington 38567), w1 dsh1 (Bloomington 5298), RhoAGFP (V318439), Rac1GFP (Bloomington 52285), cdc42GFP (V218151), w*;P{sqh-Pkn.RBD.G58A-eGFP}312a P{sqh-Pkn.RBD.G58A-eGFP}312b (Bloomington 52298), w*;P{sqh-WASp.RBD-GFP}378a P{sqh-WASp.RBD-GFP}378b (Bloomington 56746), UASmCD8GFP (Bloomington 32184), faxGFP, Sco/CyO;MKRS/TM6 (Lehmann Lab), Sco/CyO;Nos-Gal4::VP16,Vasa-KO/TM6 (Lehmann Lab); Sco/Cyo;Fz3RFP (Bach Lab).
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