UniCel DxC600 Synchron Clinical Systems (Beckman Coulter) was used for measurement of ALT, cholesterol and triglyceride levels. The level of non-esterified fatty acids was measured by the colorimetric determination reagent (Wako USA). The level of adiponectin, insulin and leptin were measured by SECTOR Imager 2400 (MSD) using the Meso Scale Discovery kits (Mouse Adiponectin Kit, Mouse Metabolic Kit for insulin and leptin, MSD). Total free testosterone levels were analyzed by enzyme immunoassay assay using a kit (CLIA Kit for Testosterone; UCSN).
Colorimetric determination reagent
Manufactured by Fujifilm
Sourced in United States
Colorimetric determination reagent is a laboratory product used to quantify the concentration of specific substances in a sample through color-based analysis. The reagent provides the necessary components to facilitate this analytical process, enabling precise measurements. The core function of the product is to support quantitative chemical analysis in various scientific and industrial applications.
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2 protocols using colorimetric determination reagent
1
Metabolic Markers in Mouse Model
2
Metabolic Markers in Mouse Model
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UniCel DxC600 Synchron Clinical Systems (Beckman Coulter) was used for measurement of ALT, cholesterol and triglyceride levels. The level of non-esterified fatty acids was measured by the colorimetric determination reagent (Wako USA). The level of adiponectin, insulin and leptin were measured by SECTOR Imager 2400 (MSD) using the Meso Scale Discovery kits (Mouse Adiponectin Kit, Mouse Metabolic Kit for insulin and leptin, MSD). Total free testosterone levels were analyzed by enzyme immunoassay assay using a kit (CLIA Kit for Testosterone; UCSN).
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