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Potassium edta

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Potassium EDTA is a chemical compound used in laboratory equipment. It is a salt of ethylenediaminetetraacetic acid (EDTA) with potassium as the counterion. Potassium EDTA is commonly used as an anticoagulant in blood collection and analysis procedures.

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Lab products found in correlation

B6c3f1 j mice, by Jackson ImmunoResearch (1 mentions) Automatic hematology analyzer, by Sysmex (1 mentions) Infinite 200 pro, by Tecan (1 mentions)

7 protocols using potassium edta

1

Rodent Blood Collection and Processing

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Rodent blood was collected under IACUC protocols for (DH) and (PL). For mice, whole blood was collected by ocular bleed. For rats, blood was collected from the saphenous vein on the inside of the thigh using a 21 gauge needle. Serum was allowed to clot at room temperature for 2–5 hours followed by centrifugation at 5000 × g for 10 minutes. The supernatant was collected and stored at −80°C in fresh tube. For plasma, blood was collected into BD 0.5ml microtainer tubes containing Potassium EDTA (Becton Dickinson, Franklin Lakes, NJ). Blood was centrifuged at 15,000 × g for 10 minutes to separate the plasma from the red blood cells. Plasma was collected, aliquoted and stored at −80°C until analysis.
Haudenschild D.R., Eldridge A., Lein P.J, & Chromy B.A. (2014). High abundant protein removal from rodent blood for biomarker discovery. Biochemical and biophysical research communications, 455(0), 84-89.
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2

Intraperitoneal ABP Dosing in Mice

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The guidelines established by the National Institutes of Health Office of Laboratory Animal Welfare were adhered to for the use of animals with housing conditions. All protocols were reviewed and approved by the University of Minnesota Institutional Animal Care and Use Committee. Eleven weeks old adult male B6C3F1/J mice (Jackson Laboratory, Bar Harbor, ME) were used in this study. The animals (N = 5) were given intraperitoneal injection of ABP at a dose of 40 mg/kg body weight (100 μL of 80% DMSO : 20% H2O). The blood was collected from the abdominal aorta into vacutainers containing potassium EDTA (Becton Dickinson), 24 h following dosing. The Hb was processed as described.35 (link)
Pathak K.V., Chiu T.L., Amin E.A, & Turesky R.J. (2016). Methemoglobin Formation and Characterization Hemoglobin Adducts of Carcinogenic Aromatic Amines and Heterocyclic Aromatic Amines. Chemical research in toxicology, 29(3), 255-269.
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3

Hematology and Transaminase Analysis in Mice

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Whole blood was obtained from the mice through their heart and collected in a potassium EDTA (Becton Dickinson, Franklin Lakes, New Jersey, USA) tube. Hematology profile was analyzed using an automatic hematology analyzer (Sysmex Corp., Japan). Transaminase enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed using the Lab kit from Chemelex, S. A. and the results were read at 340 nm wavelength on a spectrophotometer (Infinite 200 Pro, TECAN, Switzerland). The absorbance difference and the average absorbance difference were calculated and multiplied with 1750. Then, the concentration was expressed in units per liter.
Khairani A.F., Pamela Y., Oktavia N., Achadiyani A., Adipraja M.Y., Zhafira P.Y., Shalannandia W.A, & Atik N. (2022). Acute and sub-chronic oral toxicity study of purple sweet potato (Ipomoea batatas [L.] Lam) yogurt in mice (Mus musculus). Veterinary World, 15(3), 789-796.
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4

Comprehensive Blood Analysis Protocol

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On day 84, blood samples were collected by puncture of the coccygeal vessels. Samples were placed in vacutainer tubes without anticoagulant but with potassium EDTA (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). The coagulated and EDTA were analyzed or processed within 1 h after collection. The blood samples from tubes without anticoagulant were centrifuged at 3000× g for 15 min at 4 °C for serum separation and frozen at −80 °C for further analysis. White cell count was performed using a Neubauer chamber, utilizing a Thoma pipette after dilution to 1:20, using Türk solution. Serum concentration of vitamin E was determined according to the method described in [24 (link)] using liquid chromatography. Serum Se concentration was assessed using a fluorometric method, as described by Olson et al. [25 (link)]. Vitamin E analysis was performed at the Diagnostics and Clinical Analysis Laboratory in Pirassununga, and Se analysis was performed at the USP/FZEA Mineral Laboratory.
Blood serum was analyzed for glucose, total cholesterol, high- (HDL), low- (LDL), and very low-density lipoprotein (VLDL) cholesterol, and triglycerides using commercial kits (Laborlab®; Guarulhos, SP, Brazil) by an enzymatic-colorimetric method.
Saran Netto A., Silva T.H., Martins M.M., Vidal A.M., Salles M.S., Roma Júnior L.C, & Zanetti M.A. (2022). Inclusion of Sunflower Oil, Organic Selenium, and Vitamin E on Milk Production and Composition, and Blood Parameters of Lactating Cows. Animals : an Open Access Journal from MDPI, 12(15), 1968.
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5

Measurement of Glucose and Lipid Levels

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Blood samples were obtained in tubes containing 15% potassium EDTA (Becton Dickinson) for measurement of glucose and lipoprotein lipid levels and enumeration and isolation of CACs. Plasma glucose levels were measured with a glucose analyzer (2300 STAT Plus, YSI, Yellow Springs, Ohio, USA). Lipoprotein lipid levels were analyzed using an automated colorimetric assay as previously described (Joseph et al. 2011 (link)). Briefly, HDL-C was measured in the supernatant after precipitation with dextran sulfate, and LDL-C was calculated using the Friedewald equation:
[LDL-cholesterol]=[totalcholesterol]-[HDL-cholesterol]-([triglyceriders]/5)
Landers-Ramos R.Q., Corrigan K.J., Guth L.M., Altom C.N., Spangenburg E.E., Prior S.J, & Hagberg J.M. (2016). Short-term exercise training improves flow-mediated dilation and circulating angiogenic cell number in older sedentary adults. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 41(8), 832-841.
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6

Blood Collection for Nucleic Acid Isolation

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Before and after MS stimulus, 10 mL of blood were collected in red-capped tubes (no anticoagulant, Becton Dickinson, Franklin Lakes, NJ) by a phlebotomist and allowed to clot at room temperature for 30 min post draw. Tubes were then centrifuged 20 min at 2,000×g at 4 °C to precipitate cellular material. Supernatant was removed and transferred to cryotubes for RNA isolation.
An additional 4 mL of blood were collected into lavender-topped tubes (potassium EDTA, Becton Dickinson, Franklin Lakes, NJ) and transferred to separate cryotubes for DNA isolation.
All blood specimens were stored at −80 °C prior to nucleic acid isolation.
Turner A., Markey M., Le P., Reiter A., Cox C., Simmons S., Rao M.B., Altman L., Davis K., Huber D., Dufour J.S., Marras W, & Bhattacharya A. (2023). Disorientation effects, circulating small ribonucleic acid, and genetic susceptibility on static postural stability. Heliyon, 9(3), e14413.
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7

Evaluating Platelet Function in ACS Patients

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Blood samples were drawn via venipuncture after overnight fasting, 5 days after starting the treatment with 75 mg of clopidogrel, and 24 to 96 hours after performing percutaneous coronary intervention (PCI) in case of patients with ACS treated by this procedure.
For CYP2C19 genotyping, blood was collected into vacutainer tubes containing potassium EDTA as anticoagulant (Becton Dickinson, Franklin Lakes, New Jersey) and preserved at 2 C to 8 C until processing.
In order to reduce procedure-related platelet activation, the second tube was used to evaluate platelet aggregation. Impedance aggregometry was performed between 30 minutes and 2 hours after blood sampling, using whole blood samples collected into commercially available double-wall, hirudin blood tubes (Roche Diagnostics International Ltd, Rotkreuz, Switzerland).
Mărginean A., Bănescu C., Moldovan V., Scridon A., Mărginean M., Bălaşa R., Maier S., Ţăruşi M, & Dobreanu M. (2017). The Impact of CYP2C19 Loss-of-Function Polymorphisms, Clinical, and Demographic Variables on Platelet Response to Clopidogrel Evaluated Using Impedance Aggregometry. Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 23(3).
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