Sarkosyl

Manufactured by Avantor

Sourced in United States

Sarkosyl is a type of surfactant commonly used in laboratory procedures. It serves as a detergent and solubilizing agent, facilitating the extraction and separation of biological molecules.

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Lab products found in correlation

3 protocols using sarkosyl

Immunofluorescence Staining of Proteins

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Three dyes, ThT, ThS, and DAPI, and four antibodies, mouse anti-FLAG monoclonal antibody, rabbit anti-FLAG monoclonal antibody, 10 nm gold-labeled anti-mouse antibody, and mouse anti-β-tubulin antibody, were bought from Sigma-Aldrich (St. Louis, MO). Sarkosyl and Triton X-100 were purchased from Amresco (Solon, OH). Rabbit anti-SOD1 polyclonal antibody, mouse anti-SOD1 monoclonal antibody, and 10 nm gold-labeled anti-rabbit antibody were from Abcam (Cambridge, MA). Streptavidin, DyLight-405 conjugated secondary antibody and secondary antibody conjugated with Alexa 546/488 were from Invitrogen (Carlsbad, CA). Anti-dimedone polyclonal antibody was purchased from Millipore (Billerica, MA). Other chemicals were analytical grade, made in China.

Xu W.C., Liang J.Z., Li C., He Z.X., Yuan H.Y., Huang B.Y., Liu X.L., Tang B., Pang D.W., Du H.N., Yang Y., Chen J., Wang L., Zhang M, & Liang Y. (2018). Pathological hydrogen peroxide triggers the fibrillization of wild-type SOD1 via sulfenic acid modification of Cys-111. Cell Death & Disease, 9(2), 67.

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Prion Protein Analysis Techniques

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Three dyes, ThT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and DAPI, and two antibodies, the mouse anti-PrP monoclonal antibody 3F4^{41 (link),48 (link)} and a 10-nm gold-labeled anti-mouse antibody^{7 ,9 (link),57 (link)}, were purchased from Sigma-Aldrich (St. Louis, MO). Sarkosyl and PK were obtained from Amresco (Solon, OH). Alexa 546-conjugated fluorescent secondary antibody^{7 ,9 (link),57 (link)}, the proteasome inhibitor MG132, and the ROS probe DCFH-DA (2′,7′-dichlorofluorescein diacetate) were purchased from Beyotime (Nantong, China), respectively. Ni-Sepharose was obtained from GE Company (Pittsburgh, PA). All other chemicals used in this study were of analytical grade and were produced in China.

Yi C.W., Wang L.Q., Huang J.J., Pan K., Chen J, & Liang Y. (2018). Glycosylation Significantly Inhibits the Aggregation of Human Prion Protein and Decreases Its Cytotoxicity. Scientific Reports, 8, 12603.

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Purification of Amyloid Aggregates

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Congo red, thioflavin T (ThT), and guanidine thiocyanate (GdnSCN) were purchased from Sigma-Aldrich (St Louis, USA). DNA polymerase KOD-plus-Neo was from Toyobo (Tokyo, Japan). Sarkosyl and guanidine hydrochloride (GdnHCl) were obtained from Amresco (Solon, USA). Q-Sepharose Fast Flow was purchased from GE Company (Pittsburgh, USA). All other reagents used were made in China and of analytical grade. All reagent solutions used were prepared in either 10 mM NaH
₂PO
₄-H
₃PO
₄ (pH 2.5) or 50 mM MES buffer (pH 6.2) containing 120 mM NaCl unless specified otherwise.

Dang H., Chen Z., Chen W., Luo X., Liu P., Wang L., Chen J., Tang X., Wang Z, & Liang Y. (2021). The residues 4 to 6 at the N-terminus in particular modulate fibril propagation of β 2-microglobulin : N-terminal modulates β2M fibril propagation. Acta Biochimica et Biophysica Sinica, 54(2), 187-198.

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