Immobilon p polyvinylidene difluoride membrane 0.45 m

Reagents were obtained from the following sources: Torin 2 (9-(6-aminopyridine-3-yl)-1-(3-trifluromethyl)-phenyl) benzos [h] [1 (link),6 (link)] naphthyridin-2 (1 H) (cat # 4248) was obtained from Tocris Bioscience (R & D Systems, Minneapolis, MN, USA). RapaLink-1 was purchased from MCE MedChem Express, Monmouth Junction, NJ, USA, Cat No.: HY-111373. Rapamycin was obtained from Cell Signaling (cat #9904). Metformin hydrochloride (N, N-dimethyllimidodicarbonimidic diamide hydrochloride) (Tocris, cat #2864) and other chemicals were obtained either from Sigma (St. Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA). Immobilon-P polyvinylidene difluoride membrane (0.45 µm) and the reagents for enhanced chemiluminescence (ECL) were obtained from Millipore (Burlington, MA, USA) (Immobilon Western chemiluminescent horseradish peroxidase). High-performance liquid chromatography (HPLC)-grade methanol, acetonitrile, ammonium acetate, acetic acid, propylene glycol, and phosphate-buffered saline (PBS) (1×) were obtained from Fisher Scientific (Fair Lawn, NJ, USA) as previously described [17 (link)]. Isoflurane was obtained from Halocarbon Product Corporation (River Edge, NJ, USA).