Nicotinamide riboside chloride (Medkoo Biosciences, Cat# 329479) was dissolved in water (1 mg/ml) and diluted to 16 μg/L or 160 μg/L concentration in control and ethanol group’s tanks. TES-1025 (Medchemexpress, Cat# HY-111365) was dissolved in DMSO (100 mg/ml) and applied at 192 μg/L concentration for 2 days during ethanol exposure. Riboflavin (MilliporeSigma, Cat# 555682) was dissolved in 0.05 N NaOH (1 mg/ml) and treated at 60 μg/L or 600 μg/L concentration.
Nicotinamide riboside chloride
Manufactured by MedKoo Biosciences
Nicotinamide riboside chloride is a form of vitamin B3 (niacin). It is a white crystalline powder that serves as a precursor to nicotinamide adenine dinucleotide (NAD+), a coenzyme involved in various cellular metabolic processes.
Automatically generated - may contain errors
Lab products found in correlation
Ionomycin, by Selleck Chemicals (1 mentions)
Oligomycin, by Merck Group (1 mentions)
Fluoro carbonyl cyanide phenylhydrazone (fccp), by Selleck Chemicals (1 mentions)
Rapamycin, by Selleck Chemicals (1 mentions)
Bafilomycin a1, by Selleck Chemicals (1 mentions)
Ex527, by Selleck Chemicals (1 mentions)
Metformin, by Merck Group (1 mentions)
Triacsin c, by Enzo Life Sciences (1 mentions)
Palmitic acid, by Merck Group (1 mentions)
3 protocols using nicotinamide riboside chloride
1
Ethanol Exposure Modulation with NR, TES-1025, and Riboflavin
2
Mitochondrial Metabolism Modulation
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Antibodies used are listed in Supplementary Table 2 . Nicotinamide Riboside chloride (MedKoo Biosciences, #329479), EX527 (Selleck, S1541), AZD2281 (APExBIO, A4154), metformin (Sigma, PHR1084), ionomycin (Selleck, S7074), rapamycin (Selleck, S1039), Bafilomycin A1(Selleck, S1413), Oligomycin (Millipore, 3433895), FCCP (Selleck, S8276) and Antimycin A1 (MedChemExpress, HY-107406) were used at indicated concentration.
3
Reagents and Antibodies for Metabolic Study
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Palmitic acid and oleic acid-bovine serum albumin (BSA) solutions were purchased from Sigma-Aldrich (St. Louis, MO). JBSNF-000088 was purchased from MCE (Monmouth Junction, NJ), and II399 was kindly provided by Dr. Rong Huang from Purdue University. Triacsin C was obtained from ENZO life sciences (Farmingdale, NY). 2-FluoroPalmitic acid was obtained from Cayman Chemical (Ann Arbor, MI). Nicotinamide riboside chloride was purchased from MedKoo Biosciences (Morrisville, NC). The antibodies for PPARγ (No. 2443), NNMT (ab11978), and β-actin (AC026) were obtained from Cell Signaling Technology (Beverly), Abcam (Boston, MA), and ABclonal (Woburn, MA), respectively. Detailed information about reagents, antibodies, and assay kits used in this study is listed in Table 1.
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