Western Blotting was performed as in Prada et al.,57 (link) using rabbit anti-Alix (1:500; Covalab), mouse anti-Flotillin (1:1000, BD Biosciences), rabbit anti-Annexin A2 (1:5000, Abcam), rabbit anti-GAPDH (1:1000, #247002, Synaptic Systems), mouse anti-GS28 (1:1000; BD Biosciences), rabbit anti-TOM20 (1:500; Santa Cruz Biotechnology) and mouse anti-Aβ 6E10 (1:1000; Biolegend, previously Covance cat. #SIG-39300). See also Supplementary material .
Mouse anti aβ 6e10
Manufactured by BioLegend
Sourced in United States
Mouse anti-Aβ 6E10 is a monoclonal antibody that binds to a specific epitope on the amyloid-beta (Aβ) protein. It is a widely used tool in research for the detection and analysis of Aβ in various applications.
Automatically generated - may contain errors
2 protocols using mouse anti aβ 6e10
1
Western Blotting of Extracellular Vesicle Markers
2
Quantifying Alzheimer's Aβ42 in Fly Brains
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole brains of 24-day-old adult male flies were dissected in phosphate-buffered saline (PBS) buffer (1×, pH 7.4), fixed for 20 min in 4% paraformaldehyde in PBS at 4 °C, and washed 3 times with PBST buffer (0.1% Tween-20 in 1× PBS) for 10 min each. The brains were then transferred to 5% normal goat serum (diluted in 1% PBS), left for 2 h, and incubated overnight with antibodies in PBST at 4°C. The brains were then mounted and imaged on a Leica SP8 confocal microscope (Leica Co., Heissen, Germany). Mouse anti-Aβ 6E10 (1:500, BioLegend, CA, USA) was the primary antibody and the secondary antibody was rhodamine-conjugated goat anti-mouse (1:500). For Aβ42 quantification, we chose regions of interest (ROIs) in the area of cell bodies in the mushroom body, as previously reported [16] (link). Grey values of fluorescence labeling by anti-Aβ were determined after subtracting the background values, which were taken from non-immunostained areas.
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