Log In Sign up
The largest database of trusted experimental protocols

Las image analysis software

Manufactured by Leica
Visit Supplier
Sourced in Germany

LAS Image Analysis software is a comprehensive image analysis tool developed by Leica. It provides a robust set of functionalities for the processing and analysis of digital images captured using Leica microscopes and imaging systems. The software enables users to perform various image-related tasks, including measurement, quantification, and data visualization.

Automatically generated - may contain errors

Lab products found in correlation

Confocal microscope, by Leica (3 mentions) Anti α tubulin fitc antibody, by Merck Group (2 mentions) Dmi4000b, by Leica (2 mentions) Dmi3000b microscope, by Leica (1 mentions) Dmi6000 inverted microscope, by Leica (1 mentions) Sp5 inverted confocal microscope, by Leica (1 mentions) Ix51 inverted microscope, by Olympus (1 mentions) Jem 1200ex 2, by JEOL (1 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions) Confocal laser scanning microscope, by Leica (1 mentions) Dm2000, by Leica (1 mentions) Cyp2e1, by Abcam (1 mentions) Dm il led microscope, by Leica (1 mentions) Fluorescence microscope, by Nikon (1 mentions) Fluorescence microscope, by Leica (1 mentions) Tcs sp5 microscope, by Leica (1 mentions) Polyclonal guinea pig anti insulin antibody, by Agilent Technologies (1 mentions) Thioflavin s, by Merck Group (1 mentions) Stereo discovery v12, by Zeiss (1 mentions) Axiocam 506, by Zeiss (1 mentions)

Close spelling variants of this product

LAS AF image analysis software LAS EZ Application Suite image analysis software Image analysis software (LAS X). LAS v. 4.3 image analysis software LAS Image Analysis Image analysis software LAS software

20 protocols using las image analysis software

1

Stomatal Characterization in Leaf Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pretreatment of samples was performed based on the method of Li, Tang & Xu (2013) (link). Fully expanded leaves were chosen from each plantlet to observe the stomata. The abaxial and adaxial surfaces of the leaves were wiped with wet absorbent cotton fiber. Then, transparent nail polish was smeared on the two sides of the leaves. After the nail polish had air-dried and formed a membrane, transparent adhesive tape was pressed onto each leaf and was subsequently stripped off. The transparent adhesive tape was then pressed on a slide, which was treated with a neutral plastic seal and made into a temporary slide. The slides were imaged using a Leica DMI 3000B microscope (Leica Microsystems, Wetzlar, Germany). The length, width, and density of stomata were measured with Leica LAS Image Analysis software (Leica Microsystems, Wetzlar, Germany). Stomatal area = Length × Width × 3.14 × 1/4 (μm2). Stomatal density = Number/Field/Field area.
Wang W., Su M., Li H., Zeng B., Chang Q, & Lai Z. (2018). Effects of supplemental lighting with different light qualities on growth and secondary metabolite content of Anoectochilus roxburghii. PeerJ, 6, e5274.
+ Open protocol
+ Expand
2

Pronotum Length Measurement in G. texensis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immediately prior to being placed in a trial, males were weighed on a Denver Instruments TP-64 digital balance (to the nearest 0.01 g) and the pronotum length measured using a stereoscope equipped with Leica LAS image analysis software (Leica Microsystems Inc., Buffalo Grove, IL, USA). Pronotum length (the distance from the anterior to posterior edges of the pronotum at the midline) is an excellent proxy for body size inG. texensis (Kelly, Tawes & Worthington, 2014 (link)).
Kelly C.D., Telemeco M.S, & Bartholomay L.C. (2015). Are attractive male crickets better able to pay the costs of an immune challenge?. PeerJ, 3, e1501.
+ Open protocol
+ Expand
3

Immortalized Bone Marrow Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
After plating, bone marrow cells were grown to confluence and coinfected with HPV16 E6/E7 and hTERT lentiviral vectors (infection number 1). After a week the cells were split and infected again only with hTERT (infection number 2) and cultured until stabilization. Samples were observed and photographed with DMI 6000 inverted microscope (Leica Microsystems) using Leica LAS Image Analysis software (Leica Microsystems). The protocol was also reported in Miceli et al. [27 (link)].
Chiara B., Ilaria C., Antonietta C., Francesca C., Marco M., Lucia A, & Gilda C. (2014). SIRT1 Inhibition Affects Angiogenic Properties of Human MSCs. BioMed Research International, 2014, 783459.
+ Open protocol
+ Expand
4

Visualizing Nodulation Dynamics in Composite Plants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Composite plants transformed with the pMtARF3:GFP-GUS or pMtARF4a:GFP-GUS were transferred to square petri dishes (12 cm × 12 cm) containing slanted agar-Fahraeus medium. GFP fluorescence of roots was visualized with an inverted microscope (Olympus IX51) using UV light with appropriate filters for GFP. For detection of GFP and RFP fluorescence in S. meliloti inoculated roots and in nodules, confocal microscopy was performed at 5 and 9 dpi with an S. meliloti strain expressing RFP (Tian et al., 2012 (link)) using an inverted SP5 confocal microscope (Leica Microsystems). GFP and RFP were excited using 488- and 543-nm lasers, and emissions were collected from 498 to 552 nm and from 578 to 626 nm, respectively. Images were processed with the LAS Image Analysis software (Leica Microsystems).
Kirolinko C., Hobecker K., Wen J., Mysore K.S., Niebel A., Blanco F.A, & Zanetti M.E. (2021). Auxin Response Factor 2 (ARF2), ARF3, and ARF4 Mediate Both Lateral Root and Nitrogen Fixing Nodule Development in Medicago truncatula. Frontiers in Plant Science, 12, 659061.
+ Open protocol
+ Expand
5

Quantifying Liver Fibrosis via Immunohistochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols
A Leica DM microscope was used to acquire images of the sections. Portal tracts were randomly chosen in the liver, five from each slide in ×20/×40 magnification. The proportion of antibody positive area (area percent) was calculated for α-SMA with LAS Image Analysis software (Leica Microsystem). Slides were graded into three groups according to the area percent values. Grade I (mild) 0–4, Grade II (moderate) 5–9 and Grade III (severe) >10 [Figures 1–3]. The pathologist was blinded to the clinical outcome.
Ramachandran P., Unny A.K., Vij M., Safwan M., Balaji M.S, & Rela M. (2019). α-Smooth muscle actin expression predicts the outcome of Kasai portoenterostomy in biliary atresia. Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association, 25(2), 101-105.
+ Open protocol
+ Expand
6

Microscopic Analysis of Plant Nodules

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nodule tissue was processed as described in Zanetti et al. 2010 . Sections of 1-2 μm were stained with 0.04% toluidine blue and examined with an Olympus IX51 inverted microscope (OLYMPUS CORPORATION). Ultrathin sections of 70 nm were stained with uranyl acetate and lead citrate, and analyzed with a JEM 1200 EX II (JEOL, Tokio, Japan) transmission electron microscope. Confocal microscopy for subcellular localization analysis and observation of ITs were performed on N. benthamiana leaves or root segments of wild type and transgenic roots of common bean with an inverted SP5 microscope (LEICA MICROSYS-TEMS, Wetzlar, Germany) using a ×20 or a ×63 objective (numerical aperture of 0.5). GFP, RFP, mCherry and DsRed were excited using 488 nm (GFP) and 543 nm (RFP, mCherry and DsRed) lasers and emissions were collected between 500 and 550 nm (GFP), 600-660 nm (RFP), 584-660 nm (mCherry) and 578-626 nm (DsRed). Images were processed with the LAS Image Analysis software (LEICA MICROSYSTEMS).
Dalla Via V., Traubenik S., Rivero C., Aguilar O.M., Zanetti M.E, & Blanco F.A. (2017). The monomeric GTPase RabA2 is required for progression and maintenance of membrane integrity of infection threads during root nodule symbiosis. Plant molecular biology, 93(6).
+ Open protocol
+ Expand
7

Mitotic Spindle Morphology Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCT116 cells were grown on glass coverslips. After overnight incubation, the cells were treated with each individual compound for 16 h. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline for 20 min, and permeabilized with 0.2% Triton X-100 for 10 min at room temperature. The cells were then incubated with anti-α-tubulin-FITC antibody (Sigma) for 45 min, and nuclei were stained with NucRed647 for 10 min. The cells were then examined by a laser-scan confocal microscope (Leica DMI 4000 B). All images were captured with an HCX PL Apo 63x oil immersion objective. Images were processed and analyzed using Leica's LAS Image Analysis software. The percentage of mitotic cells with monopolar spindles was calculated out of a total number of a minimum of 20 mitotic cells per coverslip that were detected in different and randomly chosen microscopic fields.
Zhang W., Zhai L., Lu W., Boohaker R.J., Padmalayam I, & Li Y. (2016). Discovery of novel allosteric Eg5 inhibitors through structure-based virtual screening. Chemical biology & drug design, 88(2), 178-187.
+ Open protocol
+ Expand
8

Quantifying F-actin and Morphology Changes in mDCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-10-treated mDCs were stained with rhodamine phalloidin (Invitrogen, USA) and their F-actin structures and morphologies were examined on a confocal laser scanning microscope (Leica, Germany). Images were acquired and the F-actin contents were quantified by measuring the mean fluorescent intensities in each group. The lengths and numbers of cell membrane protrusions were also measured using Leica LAS Image Analysis software [28 (link)]. At least twenty cells in each group were selected for analysis.
Xu X., Liu X., Long J., Hu Z., Zheng Q., Zhang C., Li L., Wang Y., Jia Y., Qiu W., Zhou J., Yao W, & Zeng Z. (2017). Interleukin-10 reorganizes the cytoskeleton of mature dendritic cells leading to their impaired biophysical properties and motilities. PLoS ONE, 12(2), e0172523.
+ Open protocol
+ Expand
9

Cardiac Histology and Morphometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Paraffin-embedded 4-μm left ventricular sections were stained with H&E and picrosirius red, as described before,33 (link) or GS lectin Biotinylated Griffonia Simplicifolia Lectin I (Burlingame, CA). Morphometric analyses were performed using a microscope (Leica DM2000; Leica, Wetzlar, Germany), camera (Leica DFC295 3Mpix CMOS color), and LAS Image Analysis software (Leica). Quantifications of fibrosis via via picrosirius red staining and of cardiomyocyte area via laminin staining were performed as previously described.8 (link) Capillary density was analyzed in 8 lectin-stained sections/mouse at 40× magnification. Sections were analyzed with a capillary density assessment macro in ImageJ, from which tissue area (mm2), number of vessels, and number of vessels/mm2 were calculated.
Rech M., Kuhn A.R., Lumens J., Carai P., van Leeuwen R., Verhesen W., Verjans R., Lecomte J., Liu Y., Luiken J.J., Mohren R., Cillero-Pastor B., Heymans S., Knoops K., van Bilsen M, & Schroen B. (2018). AntagomiR-103 and -107 Treatment Affects Cardiac Function and Metabolism. Molecular Therapy. Nucleic Acids, 14, 424-437.
+ Open protocol
+ Expand
10

Immunohistochemical Staining Protocols

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical fixation and staining protocols for FAH and H&E were completed as described (47 (link)). For analysis of turboGFO (tGFP), methods for FAH staining were used except the primary antibody was rabbit anti-tGFP (Axxora) used at 1:500 overnight at 4 °C. Fixation and staining methods for hF9 immunohistochemistry were as described (30 (link)).
For serial sections the primary antibodies used were in order: glutamine synthetase (1:200), Ki67 (1:200), Cyp2E1 (1:100, Abcam ab28146), Hnf4α (1:150, Santa Cruz Bio sc-8987) and hFIX (1:400). Vector IMPress conjugated to HRP detection kit was used as the secondary antibody. Positive staining was visualized with AEC (3-amino-9-ethylcarbazole) and DAB (3,3-diaminobenzidine substrate. Microscopy was performed on a DM IL LED microscope (Leica) using Leica LAS Image Analysis Software.
Nygaard S., Barzel A., Haft A., Major A., Finegold M., Kay M.A, & Markus G. (2016). A universal system to select gene-modified hepatocytes in vivo. Science translational medicine, 8(342), 342ra79.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!