Malondialdehyde mda test kit

2FF was provided by SynChem. Inc., Elk Grove Village, IL, USA. Hydrogen peroxide was purchased from Sangon Biotech (Shanghai, China). Catalase (CAT), glutathione (GSH) and malondialdehyde (MDA) test kits were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The mitochondrial membrane potential (MMP) assay kit (JC-1 Kit), lactate dehydrogenase (LDH) kit, MTT cell proliferation and cytotoxicity kit and Mn-SOD activity kit were obtained from Beyotime Biotechnology, China. ROS Fluorescent Probe-Dihydroethidium (DHE) was purchased from Vigorousbio. CO., Ltd. (Beijing, China). Biotinylated aleuria aurantia lectin (AAL, B-1395, 1:3000) was purchased from Vectorlabs, Inc. (Burlingame, CA, USA). Antibodies against Nrf2 (ab62352, 1:1000), NQO1 (ab34173, 1:1000), keap1 (ab139729, 1:2000), NF-κB p65 (ab32536, 1:2000), NF-κB p65 (phospho S536, ab76302, 1:1000) and Nrf2 (phospho S40, ab76026, 1:2000) were from Abcam (Cambridge, UK). Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 60004, 1:5000), HO-1 (10701, 1:2000), cyclooxygenase-2 (COX-2, 66351, 1:1000), IκBα (10268, 1:1000) and Histone (10856, 1:1000) were from Proteintech (Wuhan, China).

Rabbit superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) kits, and malondialdehyde (MDA) test kits were purchased from Nanjing Jiancheng Bioengineering Institute Co., Ltd. Rabbit tumor necrosis factor-α (TNF-α) and nuclear factor of κ-light chain of enhancer-activated B cells (NF-κB) p65 ELISA kits were purchased from Shanghai Lanji Biotechnology Co., Ltd. Liver tissue homogenates were prepared in ice-cold 0.9% sodium chloride solution, then oxidative stress-related indices (MDA, SOD, CAT, and GSH-Px) and inflammatory cytokines (TNF-α and NF-κB p65) were measured in accordance with the manufacturer’s protocol.

Dendrobium was purchased from Guangzhou Fuzilin Biotechnology Co. The Bradford protein quantification kit, total antioxidant capacity (T-AOC) test kit, malondialdehyde (MDA) test kit, total superoxide dismutase (T-SOD) assay kit, and catalase (CAT) assay kit were purchased from Nanjing Jiancheng Institute of Biological Engineering; The brewer’s yeast used in the trials was obtained from our own laboratory; All other reagents were analytically pure.

The puerarin standard (purity ≥ 98%, Batch no. 110752-201514) was purchased from the National Institutes for Food and Drug Control (Beijing, China). Pue-CCMs were obtained through self-made preparation in the laboratory. Puerarin (purity of 98%) was purchased from Shaanxi Xu Ang Biological Technology Co. Ltd. (Shaanxi, China). Chromatographic-grade methanol was obtained from Thermo Fisher Scientific (USA). All other chemicals and reagents were of analytical grade, and the water was deionized and double-distilled.
ISO was purchased from Nanjing Senbeijia Biological Technology Co. Ltd. (Nanjing, China). Ethylurethanm was purchased from Shanghai Shan Pu Chemical Co. Ltd. (Shanghai, China). Propranolol was purchased from Shaanxi Yongshou Pharmaceutical Co. Ltd. (Shaanxi, China). Creatine kinase (CK), lactate dehydrogenase (LDH), total superoxide dismutase (T-SOD), and the malondialdehyde (MDA) test kit were all purchased from Nanjing Jiancheng Biological Engineering research institute (Nanjing, China). B-cell lymphoma-2 (Bcl-2) antibodies and Bcl-2 associated X protein (Bax) antibodies were both purchased from Wuhan Boshide Biological Technology Company (Wuhan, China).

Ordinarily fermented and rebiotics-fermented carrot pulps are used [35 ]. Ampicillin sodium (0.5 g/mL) is from Shanghai Shenggong Bioengineering Co. Ltd. Total cholesterol (TC) test kit, triglyceride (TG) test kit, malondialdehyde (MDA) test kit, superoxide dismutase (SOD) test kit, and reduced glutathione (GSH) test kit are from Nanjing Jiancheng Bioengineering Institute.
Electronic analysis balance is obtained from Yuyao Jinnuo Tianping Instrument Co., Ltd.; Pipette from Shanghai Thermo Fisher Scientific Instrument Co. Ltd.; Ultra-low temperature refrigerator from SANYO (Japan); and 1500-823 microplate reader from Thermo Fisher Scientific.