Bio plex pro mouse cytokine th1 th2 panel 8 plex

HQS tablets (baicalein, purity > 98%) were obtained from Shijiazhuang Zhongqi Pharmaceutical Co., Ltd. (Shijiazhuang, China). OS phosphate (Tamiflu) was obtained from Roche Pharma (Schweiz) Ltd. (Shanghai, China). Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Panel 8-Plex was purchased from Bio-Rad.

Mice were euthanized as indicated earlier, and blood was collected via heart puncture. Serum was prepared and stored at −70 °C. Using the multiplex bead immunoassay (Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Panel, 8-plex, Bio-Rad, Hercules, CA, USA), the following cytokines were examined simultaneously in the serum samples, according to the manufacturer’s protocol: interleukin (IL)-2, IL-4 IL-5, IL-10, IL-12, granulocyte macrophage-colony stimulating factor (GM-CSF), TNF-α, and IFN-γ. Data were acquired using the Bio-Plex^® MAGPIX™ Multiplex Reader (Bio-Rad, Hercules, CA, USA) and analyzed using Bio-Plex Data Pro™ Software (Bio-Rad, Hercules, CA, USA). The sensitivity of the test for individual biomolecules was 0.6 pg/mL for IL-2, 0.3 pg/mL for IL-5, 1 pg/mL for IL-10, 2.3 pg/mL for IL-12(p70), 5.6 pg/mL for GM-CSF, 1.4 pg/mL for TNF-α, and 1.2 pg/mL for IFN-γ.