Proton magnetic resonance (1H NMR) spectra and carbon magnetic resonance (13C NMR) spectra were recorded on a Varian Inova AS400 spectrometer operating at 400 MHz and 100 MHz, respectively. Proton chemical shifts are reported as parts per million (ppm) on the δ scale relative to CDCl3 (δ 7.26), CD3OD (δ 3.31) or D2O (δ 4.79). 1H NMR spectral data are tabulated in terms of multiplicity of proton absorption (s, singlet; d, doublet; dd, doublet of doublet; t, triplet; q, quartet; m, multiplet; br, broad), coupling constants (Hz), number of protons. Carbon chemical shifts are reported relative to CDCl3 (δ 77.0) or CD3OD (δ 49.0). Flash chromatography was performed with ACS grade solvents from Fisher Scientific (Hanover Park, IL). Rf values are quoted for TLC plates of thickness 0.25 mm from Whatman (Florham Park, NJ). The plates were visualized with iodine, dinitrophenylhydrazine or phosphomolybdic acid reagents. For all reactions performed in an inert atmosphere, argon was used unless otherwise specified.
Inova as400 spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The Inova AS400 spectrometer is a laboratory instrument designed for nuclear magnetic resonance (NMR) spectroscopy. It is capable of performing high-resolution NMR analysis of a wide range of samples, including organic compounds, polymers, and biological macromolecules.
Automatically generated - may contain errors
Lab products found in correlation
Tlc plate, by Cytiva (2 mentions)
Acs grade solvents, by Thermo Fisher Scientific (2 mentions)
Whatman, by Cytiva (2 mentions)
Kieselgel 60 f254, by Merck Group (2 mentions)
Rp 18 f254, by Merck Group (2 mentions)
Kieselgel 60, by Merck Group (2 mentions)
Jms 700, by JEOL (2 mentions)
Cary 50 spectrophotometer, by Agilent Technologies (1 mentions)
Esi ion source, by Agilent Technologies (1 mentions)
Fluorolog 3 spectrofluorimeter, by Horiba (1 mentions)
Spectrum one fourier transformir spectrometer, by PerkinElmer (1 mentions)
Lichroprep rp 18, by Merck Group (1 mentions)
Gcms qp2010 plus, by Shimadzu (1 mentions)
12 o tetradecanoylphorbol 13 acetate tpa, by Merck Group (1 mentions)
1 phenyl 2 thiourea ptu, by Merck Group (1 mentions)
L tyrosine, by Merck Group (1 mentions)
5 protocols using inova as400 spectrometer
1
NMR Spectroscopy Analysis Procedure
2
NMR Spectroscopy of Organic Compounds
3
Spectroscopic Characterization Methods
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nuclear magnetic resonance (NMR) spectra
were recorded with a Varian Inova AS400 spectrometer (Palo Alto) at
400 MHz (1H) and 100 MHz (13C). High-resolution
mass spectra (HRMS) were recorded with an Agilent 6210 time-of-flight
(TOF) LC–MS apparatus equipped with an ESI ion source (Agilent
Technologies, Canada). UV–visible absorption spectroscopy was
performed with a Varian Cary 50 spectrophotometer using 10 mm path
length quartz cells. Fluorescence spectra were acquired on a Fluorolog
3 spectrofluorimeter (Jobin-Yvon Horiba).
were recorded with a Varian Inova AS400 spectrometer (Palo Alto) at
400 MHz (1H) and 100 MHz (13C). High-resolution
mass spectra (HRMS) were recorded with an Agilent 6210 time-of-flight
(TOF) LC–MS apparatus equipped with an ESI ion source (Agilent
Technologies, Canada). UV–visible absorption spectroscopy was
performed with a Varian Cary 50 spectrophotometer using 10 mm path
length quartz cells. Fluorescence spectra were acquired on a Fluorolog
3 spectrofluorimeter (Jobin-Yvon Horiba).
4
Spectroscopic and Chromatographic Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Kieselgel 60 and LiChroprep RP-18 resins were used for column chromatography (Merck, Darmstadt, Germany). Kieselgel 60 F254 (Merck) and RP-18 F254S (Merck) were used as solid phases for TLC experiment. Spots on the TLC plate were detected by observing the plates under a UV lamp (Spectroline, model ENF-240 C/F; Spectronics Corp., New York, NY, USA) or by spraying 10% aqueous H2SO4 on the developed plate followed by heating. Optical rotations were measured using a JASCO P-1010 digital polarimeter (Jasco, Tokyo, Japan). A Shimadzu GCMS-QP2010 Plus (Shimadzu, Tokyo, Japan) mass spectrometer (MS) was used for gas chromatography (GC)/MS experiments. Fast atom bombardment (FAB)/MS spectrum was recorded on a spectrometer (JMS-700; JEOL, Tokyo, Japan). IR spectra were obtained from a PerkinElmer spectrum one Fourier transform-IR spectrometer (PerkinElmer, Buckinghamshire, UK). NMR spectra were recorded on a Varian Inova AS 400 spectrometer (400 MHz; Varian, Palo Alto, CA, USA).
5
Optimizing Ginseng Tyrosinase Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The leaves of hydroponic P. ginseng cultivated for 4 months in an aeroponic system were supplied by the Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Korea.
Materials Mushroom tyrosinase, L-tyrosine, 12-o-tetradecanoylphorbol-13-acetate (TPA) and 1-phenyl-2-thiourea (PTU) were all purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). The plastics used in the tissue culture were purchased from Falcon (Becton Dickinson, Franklin Lakes, NJ, U.S.A.), and the media and additives were obtained from Gibco (Grand Island, NY, U.S.A.). Kieselgel 60 and LiChroprep RP-18 resins were used for column chromatography (Merck, Darmstadt, Germany). Kieselgel 60 F 254 (Merck) and RP-18 F 254S (Merck) were used as solid phases for TLC experiment. Instrumental Analysis FAB-MS spectrum was recorded on a JEOL JMS-700 (Tokyo, Japan). NMR spectra were recorded on a Varian Inova AS 400 spectrometer (400 MHz, Varian, Palo Alto, CA, U.S.A.).
Materials Mushroom tyrosinase, L-tyrosine, 12-o-tetradecanoylphorbol-13-acetate (TPA) and 1-phenyl-2-thiourea (PTU) were all purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). The plastics used in the tissue culture were purchased from Falcon (Becton Dickinson, Franklin Lakes, NJ, U.S.A.), and the media and additives were obtained from Gibco (Grand Island, NY, U.S.A.). Kieselgel 60 and LiChroprep RP-18 resins were used for column chromatography (Merck, Darmstadt, Germany). Kieselgel 60 F 254 (Merck) and RP-18 F 254S (Merck) were used as solid phases for TLC experiment. Instrumental Analysis FAB-MS spectrum was recorded on a JEOL JMS-700 (Tokyo, Japan). NMR spectra were recorded on a Varian Inova AS 400 spectrometer (400 MHz, Varian, Palo Alto, CA, U.S.A.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!