Ff01 641 75

Manufactured by IDEX Corporation

The FF01-641/75 is a high-performance optical filter designed for use in various laboratory applications. It features a narrow bandpass filter with a center wavelength of 641 nanometers and a bandwidth of 75 nanometers. The filter is made of durable materials and is suitable for use in a range of experimental setups.

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Lab products found in correlation

Ix71 microscope, by Olympus (1 mentions) Di02 r488, by IDEX Corporation (1 mentions) Spcm aq4c, by Excelitas (1 mentions) Metamorph, by Molecular Devices (1 mentions)

2 protocols using ff01 641 75

Quantifying Nanoparticle Brightness via FCS

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FCS measurements were used to determine the average brightness per particle (BPP) of the gQD/SiO₂ before and after Au shelling. On an Olympus IX71 microscope, each sample was excited using a pulsed (10 MHz) 485 nm laser at 5 μW (PicoQuant PDL 800-B) through an Olympus 60× NA 1.2 water-immersion objective. The diffraction-limited probe volume was focused 25 μm above the coverslip into the solution. After passing through a 488 nm long-pass dichroic (Di02-R488, Semrock), the emission was filtered with a 640 nm band pass (FF01-641/75, Semrock), split by a 50/50 beam splitter (BS016, ThorLabs), and collected by two fiber-coupled single photon counting avalanche photodiodes (SPCM-AQ4C, Excelitas). The reflected and transmitted channels were cross-correlated using ALV5000 hardware correlators. The correlation function was averaged from at least three 5 second measurements. The BPP was determined by multiplying the average count rate by the amplitude of the correlation function, which is inversely equal to the number of particles in the probe volume.

Karan N.S., Keller A.M., Sampat S., Roslyak O., Arefin A., Hanson C.J., Casson J.L., Desireddy A., Ghosh Y., Piryatinski A., Iyer R., Htoon H., Malko A.V, & Hollingsworth J.A. (2015). Plasmonic giant quantum dots: hybrid nanostructures for truly simultaneous optical imaging, photothermal effect and thermometry †Electronic supplementary information (ESI) available: Further information on Au shelling chemistry and imaging of the Au shell by electron microscopy. Figures and Movie. See DOI: 10.1039/c5sc00020c. Chemical Science, 6(4), 2224-false.

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Live Cell Imaging of Fluorescent Proteins

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Live HeLa (S3) cells that expressed AzaleaB5 or AzaleaB5/I165M on 35-mm glass-bottom dishes were incubated in Hanks’ Balanced Salt Solution (HBSS) containing 15 mM HEPES-NaOH (pH 7.4) and imaged on an inverted microscope (IX-70, Olympus) equipped with a standard 75 W xenon arc lamp, a 40× objective lens (UPlanFLN 40×/1.3 NA), and a cooled CCD camera (CoolSNAP HQ2, Photometrics). The whole system was controlled with MetaMorph (Molecular Devices LLC., Sunnyvale, CA, USA). Cells were exposed to continuous unattenuated arc-lamp illumination through an Exciter: 550DF30 (Omega). At the same time, image acquisition was performed every 30 s with a short exposure time (50 ms) using a cube, which accommodated the following filters.
Exciter: 546DF10 (Omega) combined with an ND filter (10% transmittance)
Dichroic mirror: FF580-FDi01 (Semrock)
Emitter: FF01-641/75 (Semrock)
See Fig. S1.

Ando R., Sakaue-Sawano A., Shoda K, & Miyawaki A. (2023). Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Cell Structure and Function, 48(2), 135-144.

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