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Q tof premier nanoaquity

Manufactured by Waters Corporation
Sourced in United States

The Q-TOF Premier/nanoAquity is a liquid chromatography-mass spectrometry (LC-MS) system designed for high-performance analysis of complex samples. It combines a quadrupole time-of-flight (Q-TOF) mass analyzer with a nanoAquity ultra-high performance liquid chromatography (UHPLC) system. The core function of this system is to provide sensitive and accurate mass analysis of analytes separated by the UHPLC, enabling identification and quantification of small molecules, peptides, and proteins in a variety of sample types.

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3 protocols using q tof premier nanoaquity

1

Spectroscopic Analysis of Dehydroabietic Acid

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IR spectra were measured on a Nexus 870 FTIR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), and the absorption bands are expressed in cm−1. The HRMS spectra were recorded on a high-resolution mass spectrometer equipped with electrospray (ESI) and nanospray sources, and a quadrupole-time of flight hybrid analyzer (Q-TOF Premier/nanoAquity, Waters, Milford, MA). 1H NMR and 13 C NMR spectra were accomplished in CDCl3 on a Bruker AV-300, AV-500 and DRX-600 NMR spectrometer (Billerica, MA, USA) using TMS as internal standard. Reactions and the resulted products were monitored by TLC which was carried out on TLC Silica gel 60 F254 Aluminium sheets from Merck KGaA, Darmstadt, Germany and visualised in UV light (254 nm). Silica gel (3 0 0–400 mesh) for column chromatography was purchased from Qingdao Marine Chemical Factory, China. The reagents (chemicals), all being of A.R. grade, were purchased from Shanghai Chemical Reagent Company (Shanghai, China) and Energy Chemical (Shanghai, China). Disproportionated rosin was provided by Zhongbang Chemicals Co., Ltd. (Zhaoqing, China), from which dehydroabietic acid (97%) was isolated according to the published method38 .
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2

Synthesis and Characterization of Compounds

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Melting points were measured on an XT-4 apparatus (Taike Corp., Beijing, China) and were uncorrected. IR spetra were measured on a Nexus 870 FT-IR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), and the absorption bands were expressed in cm−1. The HRMS spectra were recorded on a high-resolution mass spectrometer equipped with electrospray (ESI) and nanospray sources, and a quadrupole-time of flight hybrid analyzer (Q-TOF Premier/nanoAquity, Waters, Milford, MA, USA). 1H and 13C NMR spectra were obtained in CDCl3 on a Bruker DRX-600 NMR spectrometer (Billerica, MA, USA) using TMS as internal standard. Reactions and the resulted products were monitored by TLC which was carried out on TLC Silica gel 60 F254 Aluminum sheets from Merck KGaA, Darmstadt, Germany, and visualized in UV light (254 nm). Silica gel (300–400 mesh) for column chromatography was purchased from Qingdao Marine Chemical Factory (Qingdao, China). The reagents (chemicals), all being of A.R. grade, were purchased from Shanghai Chemical Reagent Company (Shanghai, China) and Energy Chemical (Shanghai, China). Ursolic acid (95%) was bought from Jingzhu Biological Technology Co., Ltd. (Nanjing, China).
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3

Characterization of Organic Compounds

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Melting points were measured on an XT-4 apparatus (Taike Corp., Beijing, China) and were uncorrected. IR spectra were measured on a Nexus 870 FT-IR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), and the absorption bands were expressed in cm−1. The HRMS spectra were recorded on a high-resolution mass spectrometer equipped with electrospray (ESI) and nanospray sources, and a quadrupole time of flight hybrid analyser (Q-TOF Premier/nanoAquity, Waters, Milford, MA, USA). 1H and 13 C NMR spectra were obtained in CDCl3 on Bruker AV-300, AV-500 (Billerica, MA, USA) and DRX-600 NMR spectrometers using TMS as internal standard. Reactions and the resulted products were monitored by TLC which was carried out on TLC Silica gel 60 F254 Aluminium sheets from Merck KGaA, Darmstadt, Germany and visualised in UV light (254 nm). Silica gel (300 ∼ 400 mesh) for column chromatography was purchased from Qingdao Marine Chemical Factory, China. The reagents (chemicals), all being of A.R. grade, were purchased from Shanghai Chemical Reagent Company (Shanghai, China) and Energy Chemical (Shanghai, China). UA (95%) was bought from Jingzhu Biological Technology Co., Ltd. (Nanjing, China).
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