Nerolidol

Antibody against phospho-AMPK (catalog Number 2535) was acquired from Cell Signalling Technology, Inc. (Beverly, MA). Nerolidol and antibodies against AMPK (catalog Number SC-25792), Nrf-2 (catalog Number SC-13032), HO-1 (catalog Number SC-10789), and β-actin (catalog Number SC-47778) were acquired from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). A thiobarbituric acid reactive substance (TBARS) assay kit, CAT assay kit, SOD assay kit, and GPx assay kit were obtained from Cayman Chemical Co. (Ann Arbor, MI). Lipopolysaccharide (LPS; Escherichia coli, serotype 0111:B4) and other reagents were purchased from Sigma-Aldrich (St. Louis, MO).

Polyclonal rabbit anti-cyclooxygenase-2 (COX-2), anti-inducible nitric oxide synthase (iNOS), and anti-glial fibrillary acidic protein (GFAP) antibodies were purchased from Abcam, Cambridge, MA, USA. Anti-ionized calcium-binding adaptor molecule-1 (Iba-1) polyclonal rabbit antibody was procured from Wako Chemicals, Richmond, VA, USA. Polyclonal rabbit anti-tyrosine hydroxylase antibody was obtained from Novus Biologicals, Littleton, CO, USA. Alexa fluor 488 conjugated secondary goat anti-rabbit antibodies were purchased from Life Technologies, Grand Island, NY, USA. Biotinylated secondary anti-rabbit antibody was purchased from Jackson Immunoresearch, West Grove, PA, USA. Nerolidol was purchased from Santa Cruz Biotechnology Inc., CA, USA. ROT and the assay kit for reduced glutathione (GSH) and other reagents of analytical grade were purchased from Sigma-Aldrich, St. Louis, MO, USA.