Alt and ast detection kits

MF (C₁₉H₁₈O₁₁, FW = 422.34, purity ≥ 95%) was purchased from Nanjing ZeLang Medical Technology Co. Ltd. (Nanjing, China). LPS (Escherichia coli, 0111: B4), D-GalN, and ZnPP IX were obtained from Sigma (St. Louis, MO, USA). ALT and AST detection kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The bicinchoninic acid (BCA) protein assay kit was purchased from Pierce (Rockford, IL, USA). Trizol reagent was purchased from Invitrogen (Grand Island, NY, USA). AMV transcriptase, RNasin, SYBR green PCR Master Mix, and the Dual-Luciferase Reporter Assay kit were obtained from Promega (Madison, WI, USA). The tumor necrosis factor (TNF)-α ELISA kit was obtained from Bender MedSystems (Vienna, Austria). Rabbit HO-1 antibody was obtained from Abcam (Cambridge, MA, UK), rabbit β-actin antibody was purchased from Cell Signaling Technology (Boston, MA, USA), and the rat anti-mouse TLR4/MD-2 complex and rat IgG2a isotype control PE were obtained from eBioscience (San Diego, CA, USA). Lipofectamine 2000 was obtained from Invitrogen (Carlsbad, CA, USA).

Ang II was provided by Sigma (St. Louis, MO, USA). MD2 (no. ab24182), TLR4 (no. ab22048), α-SMA (no. ab5694), ICAM-1 (no. ab171123), and VCAM-1 (no. ab134047) antibodies were purchased from Abcam (Cambridge, MA, USA). GAPDH (no. sc-293335) and β-actin (sc-47778) antibodies were obtained from Santa Cruz Technology (Santa Cruz, CA, USA). ERK (9101S), p-ERK (9102S), and IκB-α (4812S) were purchased from Cell Signaling Technology (Danvers, MA, USA). ALT and AST detection kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). L6H21 was synthesized and structurally identified using MS and ¹H NMR analyses, as described in our previous report [22 (link)]. For in vivo studies, L6H21 was dissolved in 1% CMC-Na, and 1% CMC-Na also acted as the vehicle control.