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Indesign cs5

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InDesign CS5 is a desktop publishing software application developed by Adobe for creating and publishing various types of documents, including magazines, newspapers, books, and brochures. It provides users with tools for layout, typography, and image manipulation.

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Lab products found in correlation

Prism 6, by GraphPad (1 mentions) Axiovision 4, by Zeiss (1 mentions) Illustrator cs5, by Adobe (1 mentions) Image lab 3, by Bio-Rad (1 mentions) Axiovision, by Zeiss (1 mentions) Lysotracker red, by Thermo Fisher Scientific (1 mentions) Lsm 710 confocal microscope, by Zeiss (1 mentions) Mab1d4, by Developmental Studies Hybridoma Bank (1 mentions)

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InDesign (37 citations)

5 protocols using indesign cs5

1

Bioimaging Data Analysis Protocol

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Statistical analyses were performed in Prism 6.3 (GraphPad Software). Drawings were composed in Illustrator CS 5.5 (Adobe), graphs were generated in Prism 6.3, and tables and figures were assembled in InDesign CS 5.5 (Adobe). Gel and blot images were captured and exported using ImageLab 3.0 (Bio- Rad). Dark-field micrographs and videos were captured using AxioVision 4.8.2 (Carl Zeiss) and tracking analysis was performed using the Tracking module available for AxioVision from Zeiss.
Wunder EA J.r., Slamti L., Suwondo D.N., Gibson K.H., Shang Z., Sindelar C.V., Trajtenberg F., Buschiazzo A., Ko A.I, & Picardeau M. (2018). FcpB Is a Surface Filament Protein of the Endoflagellum Required for the Motility of the Spirochete Leptospira. Frontiers in Cellular and Infection Microbiology, 8, 130.
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2

Image Acquisition and Analysis Protocol

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Acquired microscope images were imported into Adobe Photoshop CS5.1
(Adobe Systems, San Jose, CA) where minimal adjustments in contrast, brightness
and sharpness were made if necessary. Bar graphs were assembled in Adobe
Illustrator (CS5.1). For the in situ hybridization analyses
shown in Figs. 3 and 4, the quality of the Nissl staining in the counterstained
sections was adequate for assigning the positions of the sampling boxes used for
quantification, as described above, however the enzymatic treatment of the
sections required for the in situ protocol significantly
diminished the quality of the Nissl staining for photographic purposes. Thus,
the Nissl stained section shown in Fig. 3A
is an untreated, surrogate section taken from a P0 rat to convey lamination and
to illustrate how we used cytoarchitecture to guide placement of sampling boxes
on the film autoradiogram. Final figure layout was achieved with Adobe InDesign
CS5.5.
Aujla P.K, & Huntley G.W. (2014). Early postnatal expression and localization of matrix metalloproteinases-2 and -9 during establishment of rat hippocampal synaptic circuitry. The Journal of comparative neurology, 522(6), 1249-1263.
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3

Starvation, DMSO, and Developmental Autophagy Analysis

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For starvation and DMSO treatments, larvae were collected 72h after egg laying, raised in fresh fly media under uncrowded conditions for 16–24 h, then transferred to treatment conditions: Starvation - 20% sucrose solution for 4h before dissection; DMSO - 5% DMSO in fresh fly media for 5h before dissection. For developmental autophagy, wandering larvae were collected 120h after egg laying. LysoTracker Red (Invitrogen) staining of live fat body was performed as described (27 (link)). Confocal imaging of GFP- and mCherry-tagged proteins was performed on fixed fat body tissues as described (2 (link)). Images were further processed with ImageJ (NIH, Bethesda, MD) or Photoshop CS5 and assembled into figures using InDesign CS5 (Adobe, San Jose CA). For punctae number analysis, nuclear regions were eliminated using Photoshop CS5 to restrain the analysis to the cytosol. Punctae were counted with the Analyze Particles feature in ImageJ.
Braden C.R, & Neufeld T.P. (2016). Atg1-independent induction of autophagy by the Drosophila Ulk3 homolog, ADUK. The FEBS journal, 283(21), 3889-3897.
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4

Quantitative Fluorescent Imaging Protocol

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Due to signal intensity differences between fluorescent reporters, the confocal microscope settings was varied accordingly to satisfy optimal dynamic range for intensity measurement and facilitate the acquisition of quality pictures. To enable accurate quantification of signal changes, we match a particular sample with its untreated control imaged in one session during which the same confocal settings are held for the sample and control. The signals are then represented as percentages, allowing for comparison of different fluorescent reporters and biological repeats. Signal intensities were measured using Fiji software (https://fiji.sc).
Images were collected using Leica SP2 or Carl Zeiss 780 and 700 confocal microscopes. Figures were processed and assembled in Photoshop and InDesign CS5 (Adobe Systems).
Nodzyński T., Vanneste S., Zwiewka M., Pernisová M., Hejátko J, & Friml J. (2016). Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components. Molecular Plant, 9(11), 1504-1519.
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5

Immunostaining and Imaging of Drosophila Nervous System

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Third instar larvae and 72- to 120-hr pupae were staged as described (Andres and Thummel 1994 (link); Bainbridge and Bownes 1981 (link)). The nervous systems of pupae and 0- to 5-d-old adults were dissected, fixed in 4% paraformaldehyde, and processed using standard protocols (Lee and Luo 1999 (link)). mAb1D4 (Van Vactor et al. 1993 (link)) (anti-Fasciclin II; anti-Fas II; 1:10) and mAb9.4A (Awasaki et al. 2000 (link)) (anti-Trio; 1:4) were obtained from the Developmental Studies Hybridoma Bank. The rabbit anti-Fas II (1:3000) was a gift from Vivian Budnik (University of Massachusetts). The rabbit anti-crustacean cardioactive peptide (anti-CCAP; 1:10,000) was a gift from John Ewer (University of Valparaiso, Chilé). Biotinylated anti-mouse and anti-rabbit IgG (1:200) were obtained from Vector Labs (cat. No. BA-9200 and BA-1000, respectively). Streptavidin Alexa Fluor 488, 546, and 568 (1:200) were obtained from Invitrogen (cat. No. S11223, S11225, and S11226, respectively). Preparations were imaged by confocal laser scanning microscopy using a Zeiss LSM 710 confocal microscope. Images were processed using ImageJ 1.46j (National Institutes of Health) and Photoshop CS5, and InDesign CS5 (Adobe).
Bates K.E., Sung C., Hilson L, & Robinow S. (2014). unfulfilled Interacting Genes Display Branch-Specific Roles in the Development of Mushroom Body Axons in Drosophila melanogaster. G3: Genes|Genomes|Genetics, 4(4), 693-706.
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