Ti sapphire infrared laser

Manufactured by Spectra-Physics

Sourced in United States

The Ti-sapphire infrared laser is a tunable solid-state laser that operates in the near-infrared region of the electromagnetic spectrum. It utilizes a titanium-doped sapphire crystal as the gain medium, allowing for a broad tuning range and high-power output.

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Lab products found in correlation

Spe 2, by Leica camera (1 mentions) Trimscope, by Miltenyi Biotec (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Multiphoton microscope, by Nikon (1 mentions) Lasersharp software, by Bio-Rad (1 mentions) Fluo 4, by Thermo Fisher Scientific (1 mentions) E600fn upright microscope, by Nikon (1 mentions)

3 protocols using ti sapphire infrared laser

Multimodal Imaging of Nanophosphor Particles

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Prior to photooxidation, LY-filled cells were imaged on a Leica SPEII using a 63× oil objective (NA 1.30).
Brain slices containing nanophosphor particles were imaged on a Radiance 2000 microscope (Bio-Rad) equipped with a Ti-sapphire infrared laser (Spectra-Physics, Santa Clara, CA, USA). The slices were initially imaged with a 10× objective (Nikon, NA 0.3; Chiyoda, Tokyo, Japan) with pulsed-mode illumination (980 nm) and then continuous wave illumination (980 nm), in order to collect nanophosphor and autofluorescence signal, respectively. Sub-regions were then imaged with a 40× objective (Nikon, NA 1.30) using continuous wave illumination at 980 nm, simultaneously collecting fluorescence and transmitted light images.

Bushong E.A., Johnson DD J.r., Kim K.Y., Terada M., Hatori M., Peltier S.T., Panda S., Merkle A, & Ellisman M.H. (2014). X-ray Microscopy as an Approach to Increasing Accuracy and Efficiency of Serial Block-face Imaging for Correlated Light and Electron Microscopy of Biological Specimens. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 21(1), 231-238.

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Ex Vivo Imaging of Mouse Testes

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For ex vivo imaging, extracted testes of TcrdH2BeGFP reporter mice were immobilised in an imaging chamber, which was flushed with oxygenated (95% O₂/5% CO₂) RPMI-1640 medium (Invitrogen) containing 1% penicillin/streptomycin, 25 mM HEPES and 5 g/litre glucose. The TriM Scope (LaVision BioTec) equipped with an upright Olympus BX51 microscope with a 203/0.95 water-immersion objective and a pulsed Ti sapphire-infrared laser (Mai Tai, SpectraPhysics) turned to 920 nm was used for imaging. The Imaris software 7.7.2 (Bitplane) was used for data analysis.

Wilharm A., Brigas H.C., Sandrock I., Ribeiro M., Amado T., Reinhardt A., Demera A., Hoenicke L., Strowig T., Carvalho T., Prinz I, & Ribot J.C. (2020). Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance. Mucosal Immunology, 14(1), 242-252.

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Two-Photon Ca2+ Imaging of Neuronal Dynamics

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Two photon Ca 2+ imaging was performed with a Bio-Rad multiphoton microscope based on a 1024 scan head installed on a Nikon E600FN upright microscope with a mounted recording chamber and a Nikon 60x NA 1.0 water-immersion objective. A Millennia V pump laser coupled to a mode-locked Ti:sapphire infra-red laser (Tsunami, Spectra Physics) was used for fluorescence excitation, tuned to 790 nm. Imaging parameters and controls were as described previously (Tonini et al., 2013) (link). In short to observe Ca 2+ transients, 20 µM Fluo-4 (Molecular Probes, Eugene, Oregon) was dissolved in the intracellular recording solution. Upon achieving the whole-cell configuration, the dye was allowed to equilibrate for 10-15 min in the proximal dendrites of the imaged neuron. Line scans images (Lasersharp software; Bio-Rad, UK) triggered by electrical stimuli delivered at the soma were collected from proximal apical processes (<50 µm from the soma) at 6 ms intervals.

Castañeda M.S., Tonini R., Richards C.D., Stocker M, & Pedarzani P. (2019). Benzamil inhibits neuronal and heterologously expressed small conductance Ca²⁺-activated K⁺ channels. Neuropharmacology, 158.

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