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Deoxynucleotide mix pcr grade

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Deoxynucleotide mix PCR grade is a reagent used in the polymerase chain reaction (PCR) process. It contains a mixture of the four deoxyribonucleotides (dATP, dCTP, dGTP, and dTTP) necessary for DNA synthesis.

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Lab products found in correlation

Ni nta resin, by Thermo Fisher Scientific (3 mentions) β mercaptoethanol β me, by Thermo Fisher Scientific (3 mentions) E coli xl 10 cells, by Agilent Technologies (3 mentions) Acetonitrile hplc grade, by Agilent Technologies (3 mentions) Pfuultra hotstart dna polymerase, by Agilent Technologies (3 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Lb broth, by Merck Group (3 mentions) Quikchange 2 site directed mutagenesis kit, by Agilent Technologies (3 mentions) Thiamine pyrophosphate, by Merck Group (2 mentions) 1 deoxy d xylulose 5 phosphate sodium salt, by Merck Group (2 mentions) Pyruvate, by Merck Group (1 mentions)

3 protocols using deoxynucleotide mix pcr grade

1

Recombinant Enzyme Production Protocol

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Thiamine pyrophosphate (TPP), pyruvate, D,L-glyceraldehyde-3-phosphate (GAP), 1-deoxy-D-xylulose-5-phosphate sodium salt, bovine serum albumin, and LB-broth were purchased from Sigma Aldrich. NADPH was purchased from Alexis Biochemical, Ni-NTA resin was purchased from Invitrogen, and β-mercaptoethanol (β-Me) was purchased from Fisher. E. coli XL-10 cells, deoxynucleotide mix PCR grade, pfuUltra Hotstart DNA polymerase, QuikChange II site directed mutagenesis kit and acetonitrile (HPLC grade) were purchased from Agilent. The DNA vectors, pET28a (+) and pET15b (+), and E. coli BL-21 B (DE3) cells were purchased from EMD Biosciences. DNA sequencing services and primers were purchased from MWG operon. All the other reagents were of the highest quality commercially available.
Handa S., Dempsey D.R., Ramamoorthy D., Cook N., Guida W.C., Spradling T.J., White J.K., Woodcock H.L, & Merkler D.J. (2018). Mechanistic Studies of 1-Deoxy-D-Xylulose-5-Phosphate Synthase from Deinococcus radiodurans. Biochemistry & molecular biology journal, 4(1), 2.
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2

Purification and Characterization of Recombinant Enzymes

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TDP, pyruvate, G3P, DXP sodium salt, bovine serum albumin, and LB broth were purchased from Sigma-Aldrich. NADPH was purchased from Alexis Biochemical, Ni-NTA resin was purchased from Invitrogen, and β-mercaptoethanol (β-Me) was purchased from Fisher. E. coli XL-10 cells, deoxynucleotide mix PCR grade, pfuUltra Hotstart DNA polymerase, QuikChange II site directed mutagenesis kit, and acetonitrile (HPLC grade) were purchased from Agilent. The DNA vectors pET28a(+) and pET15b(+) and E. coli BL-21 B(DE3) cells were purchased from EMD Biosciences. DNA sequencing services and primers were purchased from MWG operon. All the other reagents were of the highest quality commercially available.
White J.K., Handa S., Vankayala S.L., Merkler D.J, & Woodcock H.L. (2016). Thiamin Diphosphate Activation in 1-Deoxy-D-xylulose 5-Phosphate Synthase: Insights into the Mechanism and Underlying Intermolecular Interactions. The journal of physical chemistry. B, 120(37), 9922-9934.
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3

Recombinant Enzyme Production Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Thiamine pyrophosphate (TPP), pyruvate, D,L-glyceraldehyde-3-phosphate (GAP), 1-deoxy-D-xylulose-5-phosphate sodium salt, bovine serum albumin, and LB-broth were purchased from Sigma Aldrich. NADPH was purchased from Alexis Biochemical, Ni-NTA resin was purchased from Invitrogen, and β-mercaptoethanol (β-Me) was purchased from Fisher. E. coli XL-10 cells, deoxynucleotide mix PCR grade, pfuUltra Hotstart DNA polymerase, QuikChange II site directed mutagenesis kit and acetonitrile (HPLC grade) were purchased from Agilent. The DNA vectors, pET28a (+) and pET15b (+), and E. coli BL-21 B (DE3) cells were purchased from EMD Biosciences. DNA sequencing services and primers were purchased from MWG operon. All the other reagents were of the highest quality commercially available.
Handa S., Dempsey D.R., Ramamoorthy D., Cook N., Guida W.C., Spradling T.J., White J.K., Woodcock H.L, & Merkler D.J. (2018). Mechanistic Studies of 1-Deoxy-D-Xylulose-5-Phosphate Synthase from Deinococcus radiodurans. Biochemistry & molecular biology journal, 4(1), 2.
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