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Kaluza v1.3 acquisition and analysis software

Manufactured by Beckman Coulter
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Kaluza™ v1.3 is an acquisition and analysis software designed for flow cytometry applications. It provides tools for data collection, visualization, and analysis.

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Lab products found in correlation

Fluorochrome conjugated monoclonal antibodies, by BioLegend (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Kaluza acquisition and analysis software Kaluza Analysis v1.3 Kaluza Acquisition Software Kaluza Analysis Software Kaluza v1.2 software Kaluza software Kaluza

2 protocols using kaluza v1.3 acquisition and analysis software

1

Characterization of Cell Surface Markers

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The spheres were collected by gentle centrifugation and dissociated using trypsin-EDTA. The resulting single cells were then washed in PBS prior to the addition of fluorochrome-conjugated monoclonal antibodies against human CD44 (APC; clone IM7) and CD24 (PE; clone ML5) (Biolegend) for 30 min at 4 °C in the dark. The LIVE/DEAD™ Fixable Violet Dead stain (Invitrogen) was used for dead cells exclusion. The labelled cells were then washed in PBS, re-suspended in Coulter Isoton™ diluent and analysed on a Gallios™ flow cytometer using Kaluza™ v1.3 acquisition and analysis software (Beckman Coulter).
Al-Juboori S.I., Vadakekolathu J., Idri S., Wagner S., Zafeiris D., Pearson J.R., Almshayakhchi R., Caraglia M., Desiderio V., Miles A.K., Boocock D.J., Ball G.R, & Regad T. (2019). PYK2 promotes HER2-positive breast cancer invasion. Journal of Experimental & Clinical Cancer Research : CR, 38, 210.
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2

Quantifying Cellular ALDH Activity

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The AldeRed™ Detection Assay (SCR150, Merck Millipore) was used in accordance with the manufacturer’s instructions. Briefly, 2 × 105 cells were incubated with the AldeRed 588-A substrate for 40 min at 37 °C, baseline fluorescence was established using negative control samples incubated with the ALDH1 inhibitor, diethylaminobenzaldehyde (DEAB). Subsequently, cells were centrifuged, re-suspended in AldeRed assay buffer and analysed on a Gallios™ flow cytometer using Kaluza™ v1.3 acquisition and analysis software (Beckman Coulter).
Al-Juboori S.I., Vadakekolathu J., Idri S., Wagner S., Zafeiris D., Pearson J.R., Almshayakhchi R., Caraglia M., Desiderio V., Miles A.K., Boocock D.J., Ball G.R, & Regad T. (2019). PYK2 promotes HER2-positive breast cancer invasion. Journal of Experimental & Clinical Cancer Research : CR, 38, 210.
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