10 female 8 week-old Non-obese diabetic/severe combined immune deficient
gamma (NSG) mice (Jackson Laboratory, Sacramento, CA, USA) were randomly divided
into 2 groups and transplanted intravenously with CCRF-CEM-FFluc cells (5 mice
in control group: pCDH empty vector lentivirus infected; 5 mice in miR-26b
overexpressed group: pCDH-miR-26b lentivirus infected). The engraftment of
CCRF-CEM-FFluc was monitored by flow cytometry (anti-human CD45-APC antibody (BD
Biosciences, San Jose, CA, USA) and GFP on mouse retro-orbital bleeding samples.
Phospho-AKT was measured by intracellular staining flow cytometry (Cell
Signaling; #11962) on mixed mouse retro-orbital bleeding samples.
Transplanted mice underwent in vivo bioluminescence imaging at
various time points. The mice in each group were followed for survival. 2 groups
of the mice were treated and experimented under the same condition. This study
was approved by Institutional Review Board (IRB) the University of Texas MD
Anderson Cancer Center.
gamma (NSG) mice (Jackson Laboratory, Sacramento, CA, USA) were randomly divided
into 2 groups and transplanted intravenously with CCRF-CEM-FFluc cells (5 mice
in control group: pCDH empty vector lentivirus infected; 5 mice in miR-26b
overexpressed group: pCDH-miR-26b lentivirus infected). The engraftment of
CCRF-CEM-FFluc was monitored by flow cytometry (anti-human CD45-APC antibody (BD
Biosciences, San Jose, CA, USA) and GFP on mouse retro-orbital bleeding samples.
Phospho-AKT was measured by intracellular staining flow cytometry (Cell
Signaling; #11962) on mixed mouse retro-orbital bleeding samples.
Transplanted mice underwent in vivo bioluminescence imaging at
various time points. The mice in each group were followed for survival. 2 groups
of the mice were treated and experimented under the same condition. This study
was approved by Institutional Review Board (IRB) the University of Texas MD
Anderson Cancer Center.