Primary antibodies used for the detection of the α6A and α6B variants were anti-α6A [western blot (WB): 1/500, immunofluorescence (IF): 1/100] (1A10, Millipore, Etobicoke, Ontario) and anti-α6B (WB: 1/500, IF: 1/100) (6B4, Millipore). These antibodies were originally a generous gift from Dr A.Sonnenberg (Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands). Other primary antibodies used were anti-integrin β4 (WB: 1/5000, IF: 1/100) (3E1, Millipore), anti-integrin α6 (IF: 1/1000) (GOH3, Millipore), anti-β-actin (WB: 1/75 000) (C4, Millipore), anti-active-β-catenin (WB: 1/2500) (8E7, Millipore) recognizing the dephosphorylated form of β-catenin on Ser37 and Thr41 (sites of GSK3β phosphorylation), anti β-catenin (WB: 1/2500) (610153, BD Biosciences, Mississauga, Ontario), anti-DVL2 (WB: 1/2500) (30D2, Cell Signaling Technology, Danvers, MA), anti-cytokeratin 18 (WB: 1/1 000 000) (CY-90, Sigma–Aldrich, Oakville, Ontario), anti-histone H1 (WB: 1/1000) (AE-4, Santa Cruz Biotechnologies, Santa Cruz, CA), anti-integrin β1 (WB: 1/1000) (Mab13, BD Biosciences), anti-GSK3β (WB: 1/5000) (27C10, Cell Signaling Technology) and anti-H3K27me3 (WB: 1/1000) (07-449, Millipore). The pharmacological inhibitor of GSK3β (SB216763, S3442, Sigma–Aldrich) was used at a final concentration of 20 µM. The protease inhibitor cocktail (P8340) was purchased from Sigma.
Sb216763 s3442
Manufactured by Merck Group
Sourced in United States
SB216763 (S3442) is a small molecule inhibitor that targets and inhibits the activity of glycogen synthase kinase-3 (GSK-3). GSK-3 is a serine/threonine protein kinase involved in various cellular processes. The core function of SB216763 (S3442) is to modulate GSK-3 activity, which can be used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Cy 90, by Merck Group (1 mentions)
Mab13, by BD (1 mentions)
Phospho gsk 3β ser9, by Cell Signaling Technology (1 mentions)
Kt5823, by Merck Group (1 mentions)
Beclin 1, by Novus Biologicals (1 mentions)
Sqstm1 p62, by Cell Signaling Technology (1 mentions)
Phospho gsk 3β, by Abcam (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Tcf1 2203 s, by Cell Signaling Technology (1 mentions)
Sc 47778 hrp, by Santa Cruz Biotechnology (1 mentions)
Cd45ra pe cy7, by BioLegend (1 mentions)
Dusp6 ab76310, by Abcam (1 mentions)
Cd69 percp cy5.5 560738, by BioLegend (1 mentions)
Cd62l pe 304806, by BioLegend (1 mentions)
Anti phosphoserine threonine tyrosine antibody, by Abcam (1 mentions)
Alexa 594, by Thermo Fisher Scientific (1 mentions)
α tubulin, by Merck Group (1 mentions)
Protein a g coated sepharose beads, by Santa Cruz Biotechnology (1 mentions)
Gsk 3β, by Merck Group (1 mentions)
Secondary antibodies conjugated to alexa 488, by Thermo Fisher Scientific (1 mentions)
4 protocols using sb216763 s3442
1
Antibody Detection of Integrin Variants
2
Autophagy Regulation Signaling Pathway
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3‐methylamphetamine (M9281), rapamycin (37094), SB216763 (S3442), and KT‐5823 (K1388) were purchased from Sigma‐Aldrich (St. Louis, MO, USA); antibodies were used against light‐chain 3B (LC3B; Aviva Systems Biology Cat# ARP51335_P050, https://scicrunch.org/resources/Any/search?q=AB_2044956&l=AB_2044956 ), Beclin 1 (Novus Cat# NB500‐249B, https://scicrunch.org/resources/Any/search?q=AB_1216268&l=AB_1216268 ), GSK‐3β (Cell Signaling Technology Cat# 12456, https://scicrunch.org/resources/Any/search?q=AB_2636978&l=AB_2636978 ), phospho‐GSK‐3β (Ser9; Cell Signaling Technology Cat# 14310, https://scicrunch.org/resources/Any/search?q=AB_2798445&l=AB_2798445 ), phospho‐GSK‐3β (Tyr216; Abcam, Cat# 75745), SQSTM1/p62 (Cell Signaling Technology Cat# 5114, RRID:AB_10624872), APG5L/ATG5 (Abcam Cat# ab109490, https://scicrunch.org/resources/Antibodies/search?q=AB_10861245&l=AB_10861245 ), https://scicrunch.org/resources/Antibodies/search?q=AB_1063421&l=AB_1063421 (LifeSpan Cat# LS‐C42463‐50, RRID:AB_1063421), https://scicrunch.org/resources/Antibodies/search?q=AB_10800793&l=AB_10800793 (LifeSpan Cat# LS‐C121392‐50, RRID:AB_10800793), https://scicrunch.org/resources/Antibodies/search?q=AB_2263076&l=AB_2263076 (Proteintech Cat# 10494‐1‐AP, RRID:AB_2263076), and β‐actin (Proteintech, Cat# 60008‐1‐Ig).
3
Immunoblotting and ChIP-PCR Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies specific for TCF1 (#2203 S) and pERK (T202/Y204, #4377 S) from Cell Signaling Technology, and DUSP6 (ab76310) from Abcam, were used at a dilution of 1 in 1000 for immunoblotting or 1 in 50 for ChIP-PCR. Antibodies specific for β-actin were from Santa Cruz Biotechnology (sc-47778 HRP) and used at 1 in 10000. Fluorochrome-conjugated antibodies were from BD Biosciences including CD4-APC (555349), CD69- PerCP-Cy5.5 (560738) and CD45RA-PE-Cy™7 and BioLegend including CD62L-PE (304806), CD25-APC (302610) and human IL-2 Alexa-Fluor-488 (500314). All antibodies for flow cytometry were used at 1 in 50. PIM1 inhibitor (526521), GSK3β inhibitors, BIO (2’Z,3’E)-6-Bromoindirubin-3ʹ-oxime (361550) and SB216763 (S3442), were from Sigma.
4
Investigating Neurodegeneration Mechanisms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An MTT assay kit was purchased from Roche. A site-directed mutagenesis kit was purchased from Stratagene. 6-Hydroxydopamine hydrobromide (6-OHDA), DMSO, SB216763 (S3442, an inhibitor of GSK3β) and AGK2 (A8231, an inhibitor of SIRT2) were obtained from Sigma-Aldrich. Antibodies against pGSK3β (Ser9) and GSK3β were purchased from Cell Signaling (Danvers, MA, USA). Antibodies against SIRT2, ace-tubulin, α-tubulin, HA and Flag were purchased from Sigma-Aldrich. Secondary antibodies conjugated to Alexa 488 or Alexa 594 were purchased from Invitrogen. Hoechst 33258 (94403) was purchased from Sigma-Aldrich. Protein A/G-coated Sepharose beads were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). An anti-phosphoserine/threonine/tyrosine antibody was obtained from Abcam (ab15556). Protein kinase CDK5/p25 (cat. 14—516) and GSK3β (cat. 14—306) were purchased from Millipore. Cells were transfected using Lipofectamine 2000 Transfection Reagent (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Other chemicals and reagents were of the highest analytical grade and were purchased from local commercial sources.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!