Primary antibodies used in this study include: goat-anti-mouse Sema6A (R&D systems, 1:200), rabbit anti-GFP (Life Technologies, IgG fraction, 1:1000), rabbit anti-GFP (Life Technologies, serum, 1:1000), chicken anti-GFP (AVES, 1:1000), rabbit anti-CART (Phoenix Pharmaceuticals Inc., 1:1000), goat anti-ChAT (Millipore, 1:200), TO-PRO3 (Life Technologies, 1:500), rabbit anti-cleaved Caspase 3(Asp 175) (5A1E) (Cell Signaling Technology, 1:200), rabbit anti-Brn3b (gift from Dr. Jeremy Nathans, 1:200), rabbit anti-plexinA2 (gift from Dr. Fumikazu Suto, 1:200), Armenian hamster anti-plexinA4 (gift from Dr. Fumikazu Suto, 1:200), and mouse anti-TujIII (Promega, 1:1000).
Rabbit anti cleaved caspase 3 asp 175 5a1e
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-cleaved Caspase 3(Asp 175) (5A1E) is a primary antibody that specifically recognizes the large fragment of activated Caspase-3 resulting from cleavage adjacent to Asp175. Caspase-3 is a key effector of apoptosis, and its activation is a hallmark of cells undergoing programmed cell death.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti chat, by Merck Group (1 mentions)
Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions)
Rabbit anti cart, by Phoenix Pharmaceuticals (1 mentions)
Alexa fluor 647 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
Rabbit anti vcam 1, by Cell Signaling Technology (1 mentions)
3 protocols using rabbit anti cleaved caspase 3 asp 175 5a1e
1
Immunohistochemical Markers in Neuroscience
2
Drosophila Immunolabeling Protocol
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Immunohistochemistry was performed following the protocol for adult Drosophila immunolabelling according to Purice et al. (2017) (link). For BrdU detection, dissected VNCs were treated with 2 M HCl for 20 min (RT) prior to incubation with anti-BrdU.
We used the following antibodies: mouse anti-Repo (DSHB, 8D12 ID: AB_528448, 1:200), rat anti-BrdU (Abcam 1:500, ab6326), mouse anti-nc82 (anti-Bruchpilot; DSHB 1:30, 2ea ID: AB_2314866), rabbit anti-cleaved caspase-3 (Asp175) (5A1E) (Cell Signaling Technology, 1:50, #9664), mouse anti-Mmp1 (DSHB, 3A6B4 ID: AB_579780; 3B8D12 ID: AB_579781; 5H7B11 ID: AB_579779 used at 1:1:1, 1:50), mouse anti-Relish-C (DSHB, 21F3 ID: AB_1553772, 1:50), guinea pig anti-Repo [gift from B. Altenhein (von Hilchen et al., 2013 (link))], rabbit anti-Drpr [gift from M. Freeman (Freeman et al., 2003 (link))], guinea pig anti-grnd [gift from P. Leopold (Andersen et al., 2015 (link))].
We used the following antibodies: mouse anti-Repo (DSHB, 8D12 ID: AB_528448, 1:200), rat anti-BrdU (Abcam 1:500, ab6326), mouse anti-nc82 (anti-Bruchpilot; DSHB 1:30, 2ea ID: AB_2314866), rabbit anti-cleaved caspase-3 (Asp175) (5A1E) (Cell Signaling Technology, 1:50, #9664), mouse anti-Mmp1 (DSHB, 3A6B4 ID: AB_579780; 3B8D12 ID: AB_579781; 5H7B11 ID: AB_579779 used at 1:1:1, 1:50), mouse anti-Relish-C (DSHB, 21F3 ID: AB_1553772, 1:50), guinea pig anti-Repo [gift from B. Altenhein (von Hilchen et al., 2013 (link))], rabbit anti-Drpr [gift from M. Freeman (Freeman et al., 2003 (link))], guinea pig anti-grnd [gift from P. Leopold (Andersen et al., 2015 (link))].
3
Immunofluorescence Staining of Frozen Liver Sections
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Frozen liver sections (10µm) were incubated with rabbit anti-VCAM-1 (1:200) or rabbit anti-cleaved caspase-3 Asp175 (5A1E) (1:100, Cell Signalling Technology USA) and sheep anti-von Willebrand Factor (vWF; 1:100) (Abcam, MA, USA) overnight at 4°C. Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) and Alexa Fluor 488 Donkey anti-Sheep IgG (H+L) (1:900; Thermo Fisher Australia) were used as secondary antibodies. Images were captured using the Nikon TiE microscope (Monash Micro Imaging Platform).
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