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2 protocols using agilent series device

1

Phytochemical Analysis of Medicinal Plants

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All of the used chemicals were of analytical grade. Absolute ethanol, methanol, n-hexane, sodium hydroxide, ethyl acetate, and dichloromethane were purchased from BDH England. Aluminium chloride and FeSO4•7H2O, silica gel was purchased from Merck Germany. Sodium carbonate and hydrogen peroxide were purchased from Unichem India. Ascorbic acid, gallic acid, rutin, Folin-Ciocalteau reagent, EDTA, ferrozine, and DPPH were purchased from Sigma Aldrich, USA. PBS was purchased from Gibco, UK. MS spectra were recorded on an Agilent series device and MSMS spectra were recorded on a Shimadzu series device.
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2

Analytical Chemical Analysis of Compounds

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The chemicals used were all of analytical grade. Absolute ethanol, methanol, n-hexane, sodium hydroxide, ethyl acetate, and dichloromethane were purchased from BDH England. Aluminium chloride, FeSO4·7H2O, and silica gel were obtained from Merck Germany. Sodium carbonate and hydrogen peroxide were obtained from Unichem India. DPPH were obtained from Sigma Aldrich, USA. PBS was obtained from Gibco, UK. MS spectra were recorded on an Agilent series device, and MSMS spectra were recorded on a Shimadzu series device.
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