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2 protocols using anti erk

1

Berberine and Cisplatin Synergistic Assay

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Berberine (purity: ≥98%) and cisplatin (purity: ≥99%) were purchased from Sigma Aldrich (St. Louis, MO, USA). Anti-MMP-2, anti-MMP-9, anti-Bcl-2, anti-Bax, anti-CyclinD1, anti-CDK4, anti-JNK, anti-phospho-JNK, anti-ERK, anti-phospho-ERK, anti-P38, and anti-phospho-P38 primary antibodies were purchased from Bioss (Beijing, China). Goat antimouse IgG and goat antirabbit IgG secondary antibodies were purchased from Life Science (Santa Cruz, CA, USA).
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2

VIP-Induced Osteoclastogenesis Regulation

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VIP was purchased from Nanjing Peptide Biotechnology Inc. (Nanjing, China). Fetal bovine serum (FBS), a modification of Minimum Essential Medium (a-MEM), and L-glutamine were supplied by Invitrogen (Carlsbad, USA). Oligonucleotide primers were ordered from Sangon Biotech (Shanghai, China). TaqMan Universal PCR Master Mix and probes were obtained from Applied Biosystems (Foster City, CA). anti-RANKL, anti-RANK, anti-OPG, anti-NF-κB, anti-interleukin 6 (IL-6), anti-ERK, anti-carbonic anhydrase II (CAII), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) primary, and anti-IgG-horseradish peroxidase secondary antibodies were purchased from Bioss Biotechnology, Inc. (Beijing, China).
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