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C myc 9e10 and c33

Manufactured by Santa Cruz Biotechnology

C-Myc (9E10 and C33) is a mouse monoclonal antibody that recognizes the c-Myc protein. The c-Myc protein is a transcription factor that plays a crucial role in cell growth and proliferation. The 9E10 and C33 clones of the C-Myc antibody are commonly used in research applications to detect and study the expression and localization of the c-Myc protein.

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2 protocols using c myc 9e10 and c33

1

Antibody Information for Western Blot

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Antibodies used in this study include N-Myc (OP-13; 1:1000) from EMD Biosciences; p53 (DO-1; 1:1000), and c-Myc (9E10 and C33; 1:500) from Santa Cruz Biotechnology; Ki-67 (SP6; 1:500) from Lab Vision; β-actin (AC-15; 1:10,000), Vinculin (V284; 1:1000), α-Tubulin (B-5-1-2; 1:4000) and Flag (M2; 1:1000) from Sigma; p53 (CM5; 1:1000) from Leica Microsystems; HA (3F10; 1:1000) and BrdU (1:500) from Roche Applied Science; GFP (JL-8; 1:1000) from Clontech; N-Myc (9405; 1:500), Lamin B1 (12586; 1:1000), and Parp1 (9532; 1:1000) from Cell Signaling; For IHC, HAUSP (1:500) Bethyl; For westerns, HAUSP antibody as described before5 (link) (1:1000). All primary antibodies were diluted in 5% (w/v) nonfat dry milk in Tris-buffered saline with 0.1% Tween-20, except N-Myc (9405) which was diluted in 5% (w/v) BSA in Tris-buffered saline with 0.1% Tween-20.
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2

Antibody Information for Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study include N-Myc (OP-13; 1:1000) from EMD Biosciences; p53 (DO-1; 1:1000), and c-Myc (9E10 and C33; 1:500) from Santa Cruz Biotechnology; Ki-67 (SP6; 1:500) from Lab Vision; β-actin (AC-15; 1:10,000), Vinculin (V284; 1:1000), α-Tubulin (B-5-1-2; 1:4000) and Flag (M2; 1:1000) from Sigma; p53 (CM5; 1:1000) from Leica Microsystems; HA (3F10; 1:1000) and BrdU (1:500) from Roche Applied Science; GFP (JL-8; 1:1000) from Clontech; N-Myc (9405; 1:500), Lamin B1 (12586; 1:1000), and Parp1 (9532; 1:1000) from Cell Signaling; For IHC, HAUSP (1:500) Bethyl; For westerns, HAUSP antibody as described before5 (link) (1:1000). All primary antibodies were diluted in 5% (w/v) nonfat dry milk in Tris-buffered saline with 0.1% Tween-20, except N-Myc (9405) which was diluted in 5% (w/v) BSA in Tris-buffered saline with 0.1% Tween-20.
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