Hrp conjugated goat anti mouse igg or iga

Manufactured by Southern Biotech

HRP-conjugated goat anti-mouse IgG or IgA is a secondary antibody used to detect and quantify mouse immunoglobulins (IgG or IgA) in various immunoassays. The antibody is conjugated with the enzyme horseradish peroxidase (HRP), which enables colorimetric or chemiluminescent detection of the target immunoglobulins.

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Lab products found in correlation

Immuno plate, by Thermo Fisher Scientific (1 mentions) Synergy h1 hybrid reader, by Agilent Technologies (1 mentions) Elisa plate, by BD (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Elisa reader, by Molecular Devices (1 mentions)

Close spelling variants of this product

Goat anti-mouse IgA (26 citations) Anti-mouse IgA (14 citations) Goat anti-mouse IgA-HRP (9 citations) Anti-mouse IgA-HRP (9 citations) HRP-conjugated goat anti-mouse IgA (8 citations) HRP-conjugated goat anti-mouse (7 citations) Goat anti-mouse HRP (5 citations) HRP-conjugated goat anti-mouse IgG or anti-mouse IgA (2 citations)

3 protocols using hrp conjugated goat anti mouse igg or iga

ELISA for VP1 Antibody Detection

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Plasma and fecal extracts were assayed for the presence of antibodies against VP1 antigen by an ELISA method. Plasma samples were tested at a dilution of 1:10 and HRP-conjugated goat anti-mouse IgG diluted 1:1000 was used as a secondary antibody. Fecal extracts were analyzed undiluted and secondary labeling was done using HRP-linked goat anti-mouse IgA at 1:1000 dilution. Plates were coated with 10 μg/ml VP1_237–249 peptide (GL Biochem Corp, Shanghai) at 4 °C overnight. After blocking with 5% non-fat milk in PBS, serum or fecal samples were added and incubated at 37 °C for 2 h. After washing the plates three times, HRP-conjugated goat anti-mouse IgG or IgA (Southern-Biotech) was added, followed by TMB substrate addition. After incubation for 30 min at room temperature, the reaction was stopped by the addition of 0.5 M sulfuric acid and the absorbance was quantified at 450 nm by a microplated reader (Bio-Lab).

Qi X., Lu Q., Hu J, & Xiong S. (2019). Spontaneous C-cleavage of a truncated intein as fusion tag to produce tag-free VP1 inclusion body nanoparticle vaccine against CVB3-induced viral myocarditis by the oral route. Microbial Cell Factories, 18, 66.

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Measuring IgA and IgG Antibodies in Murine Samples

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Serum and feces samples were obtained from OVA injected mice. Feces were suspended as 100 mg/ml with PBS containing 0.02% sodium azide, stirred vigorously and centrifuged 8,000 rpm for 10 mins. The supernatant was used for determination of antigen-specific secretary IgA. Immunoplates (Thermo Fisher Scientific, Roskilde, Denmark) were coated with 10 µg/ml OVA or flagellin in 0.05 M carbonate-bicarbonate buffer (pH 9.6) and incubated overnight at 4°C. Wells were blocked with 1% bovine serum albumin in PBS. Diluted serum or fecal samples were added to the wells. Plates were then treated with HRP-conjugated goat anti-mouse IgG or IgA (Southern Biotech, Birmingham, AL) and incubated overnight at 4°C. Peroxidase substrate TMB (MOSS Inc., Pasadena, MD) was added and the reaction was stopped with 0.5 N HCl. Plates were read at 450 nm by ELISA reader (Synergy H1 Hybrid Reader, BioTek, Winooski, VT). Endpoint titers were determined as the reciprocal log₂ of the last dilution giving an OD at 450 nm of 0.1 greater than background.

Kim Y.I., Song J.H., Ko H.J., Kweon M.N., Kang C.Y., Reinecker H.C, & Chang S.Y. (2018). CX3CR1+ macrophages and CD8+ T cells control intestinal IgA production. Journal of immunology (Baltimore, Md. : 1950), 201(4), 1287-1294.

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ELISA for Detecting Antibody Responses

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ELISA plates (Falcon, Franklin Lakes, NJ) were coated with mOMVs, waaJ-mOMVs, and wtOMVs, respectively, in PBS and incubated overnight at 4°C. Blocking was performed with 1% BSA (Sigma-Aldrich) in PBS, and 2-fold serial sample dilutions were applied to plates. HRP-conjugated goat anti-mouse IgG or IgA (Southern Biotechnology Associates, Birmingham, AL) was added to each well and incubated overnight at 4°C. A tetramethylbenzidine solution (Moss, Pasadena, MD) was used for color development. Plates were measured at 450 nm using an ELISA reader (Molecular Devices, Sunnyvale, CA) after the addition of stopping solution (0.5 N HCl).

Choi K.S., Kim S.H., Kim E.D., Lee S.H., Han S.J., Yoon S., Chang K.T, & Seo K.Y. (2014). Protection from Hemolytic Uremic Syndrome by Eyedrop Vaccination with Modified Enterohemorrhagic E. coli Outer Membrane Vesicles. PLoS ONE, 9(7), e100229.

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