Gsk1016790a

The pharmacological and small‐molecule inhibitors and agonists used were the following: 7rh (2.5 µm; Sigma) for DDR1 inhibition, rat anti‐human CD29 (1:100; BD Bioscience) to block integrin β1, blebbistatin (1 µm; Enzo Life Sciences) for myosin II inhibition, GM6001 (10 µm; Calbiochem) as broad‐spectrum MMP inhibitor, L‐mimosine (400 µm; Santa Cruz Biotechnology) to arrest the cell cycle at G1, S‐trityl‐L‐cysteine (STLC; 20 µm; Santa Cruz Biotechnology) to arrest at mitosis, ionomycin (10 µm; MedChemExpress) to increase cell membrane permeability to calcium, gadolinium chloride (10 µm; MedChemExpress) to inhibit mechanosensitive calcium channels, BAPTA (10 µm; MedChemExpress) to chelate extracellular calcium, BAPTA‐AM (10 µm; MedChemExpress) to chelate intracellular calcium, arachidonic acid (70 µm; MedChemExpress) to mimic signaling downstream of nuclear mechanosensing, AACOCF3 (28 µm; Tocris) to inhibit signaling downstream of nuclear mechanosensing, 2‐aminoethoxydiphenylborane (20 µm; MedChemExpress) as IP3 receptor agonist, Xestospongin c (10 µm; Tocris) as IP3 receptor agonist, GSMT×4 (10 µm; MedChemExpress) as Piezo 1 inhibitor, Yoda1 (6 µm; MedChemExpress) as Piezo 1 agonist, GSK205 (10 µm; MedChemExpress) as TRPV4 inhibitor, and GSK1016790A (50 µm; MedChemExpress) as TRPV4 agonist.

Prostaglandin E₂, Serotonin hydrochloride(5-HT), SKF-96365, TPEN (N, N, N′, N′- tetrakis (2-pyridylmethyl) ethylenediamine), GSK-7975A, ouabain, HC067047, and GSK1016790A were purchased from MedChemExpress (MCE; Monmouth Junction, NJ, United States). Sigma (Saint Louis, MO, United States) supplied carbamylcholine chloride (CCh), nifedipine, gadolinium chloride, and cyclopiazonic acid (CPA). Tocris Bioscience (Ellisville, MO, United States) supplied 2-Aminoethoxydiphenyl borate (2-APB), while APExBIO Technology LLC (Houston, TX, United States) provided dantrolene. Fura-2 was purchased from Invitrogen (UT, United States), meantime DMEM and FBS were obtained from Hyclone (Logan, UT, United States). Trypsin and penicillin/streptomycin were purchased from Gibco (CA, United States). The other chemicals were obtained from BBI Life Science (Shanghai, China).