Nkg2a

Manufactured by Miltenyi Biotec

NKG2A is a cell surface receptor expressed on natural killer (NK) cells and a subset of T cells. It functions as an inhibitory receptor, playing a role in the regulation of NK cell and T cell activity.

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Lab products found in correlation

Tigit, by Thermo Fisher Scientific (2 mentions) Bcl 2, by BioLegend (1 mentions) Ki 67, by Miltenyi Biotec (1 mentions) Kir2dl1 s1, by Beckman Coulter (1 mentions) Nkg2c, by Miltenyi Biotec (1 mentions) Nkg2c, by R&D Systems (1 mentions) Cd122, by BD (1 mentions) Lag 3, by Miltenyi Biotec (1 mentions) Kir2dl1, by R&D Systems (1 mentions) Kir2dl3, by R&D Systems (1 mentions) Igg2a, by BioLegend (1 mentions) Dnam 1, by BD (1 mentions) Klrg1, by Thermo Fisher Scientific (1 mentions) Facscanto flow cytometer, by BD (1 mentions) Perforin, by BD (1 mentions) Tim 3, by BD (1 mentions) Lag 3, by BD (1 mentions) Cd107a, by BD (1 mentions) Anti cd3, by R&D Systems (1 mentions) Nkg2d, by BD (1 mentions)

3 protocols using nkg2a

Comprehensive Immunophenotyping Protocol

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The following antibodies were used for flow cytometry: BD Bioscience: CD3 epsilon (UCHT1, SP34–2 and SK7), CD122 (Mik-β3), CD8a (RPA-T8), PLZF (RI7–809). CD107a (H4A3), IFN-γ (B27), KIR3DL1 (DX9), KIR2DL2/L3/S2 (CH-L), CD16 (3G8), CD57 (NK-1), HLA-DR (G46–6), HLA-C (DT9), and CD86 (FUN-1). Biolegend: CD2 (RPA-2.10), PD-1 (EH12.2H7), Bcl-2 (100), CD3 epsilon (SK7, OKT3, HIT3a and APA1/1), HLA-ABC (W6/32), NKp46 (9E2), IgG1 (MOPC-21), IgG2a (MOPC-173), CD5 (UCHT2), Lck (LCK-01), and Bcl11b (25E6). Beckman Coulter: CD56 (NKH-1), KIR2DL2/L3/S2 (GL183), KIR2DL1/S1 (EB6), NKp46 (BAB281), CD247 (TIA-2), CD69 (TP1.55.3). Miltenyi: NKG2A (REA110), NKG2C (REA205), PD-1(PD1.3.1.3), LAG-3 (REA351), CD58(TS2/9), Ki-67 (REA183), Bw4 (REA274), and TCRrd (11F2). R&D Systems: NKG2C (134591), KIR2DL1 (143211), and KIR2DL3 (180701). Millipore: FcεRI (FCABS400F), HCMV-IEA (8B1.2). ThemoFisher: LIR-1 (HP-F1), TCRab (WT13), and CD8b (SIDI8BEE). Abcam: CD3 gamma (EPR4517), CD3 delta (EP4426) and Bcl11b (25E6). Jackson Immunoresearch: Donkey anti-rabbit IgG (H+L). The following antibodies were used for western blot: Biolegend: CD3 epsilon (SK7), IgG1 (MOPC-21), β-actin (W16197A), CD247 (6B10.2), pLck (A18002D), Lck (LCK-01), CD2 (RPA-2.10), and CD16 (3G8); pCD247 (C415.9A, Santa Cruz Biotechnology), CD3 gamma (EPR4517, Abcam), and CD3 delta (EP4426, Abcam).

Wu Z., Lau C.M., Sottile R., Le Luduec J.B., Panjwani M.K., Conaty P.M., Srpan K., Laib Sampaio K., Mertens T., Adler S.P., Hill A.B., Barker J.N., Cheung N.K., Sun J.C, & Hsu K.C. (2021). Human Cytomegalovirus Infection Promotes Expansion of a Functionally Superior Cytoplasmic CD3+ NK Cell Subset with a Bcl11b-Regulated T Cell Signature. The Journal of Immunology Author Choice, 207(10), 2534-2544.

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Comprehensive Immune Phenotyping of Blood Samples

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Blood samples (100 µl) collected from the participants were incubated for 25 min at 25 °C in a flow cytometry tube with the following specific antibodies (5 µl each): CD3 (563219, BD), CD16 (302018, Biolegend), CD56 (555518, BD), TIGIT (12-9500-42, eBioscience), DNAM-1 (559788, BD), NKG2A (130-113-565, Miltenyi Biotec), LAIR-1 (ab27744, Abcam), Siglec-7 (ab200558, Abcam), and KLRG1 (25–9488-42, eBioscience). In addition, an isotype control was prepared for each sample. After incubation, the reaction mixture in each tube was mixed thoroughly with 2 ml of hemolysin. The samples were then washed for flow cytometry analysis (BD FACSCanto™ Flow Cytometer) within 4 h.

Nie Y., Liu D., Yang W., Li Y., Zhang L., Cheng X., Chen R., Yuan B., Zhang G, & Wang H. (2022). Increased expression of TIGIT and KLRG1 correlates with impaired CD56bright NK cell immunity in HPV16-related cervical intraepithelial neoplasia. Virology Journal, 19, 68.

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Multiparametric Immune Cell Profiling

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Fluorochrome-conjugated antibodies were purchased from BioLegend (CD3 [catalog 317330], CD56 [catalog 362510], NKG2D [catalog 320806], CD96 [catalog 338418], granzyme B [catalog 515408], perforin [catalog 308112], IFN-γ [catalog 502538], TNF-α [catalog 502932], CD107a [catalog 328606], TIM-3 [catalog 345018], LAG-3 [catalog 369320], Ki67 [catalog 350515], human CD45 [catalog 304041], mouse CD45 [catalog 103149]); BD Biosciences (anti-CD3 [catalog 562406], CD16 [catalog 560717]); R&D Systems (TIGIT [catalog FAB7898A], TRAIL [catalog FAB687G]); Invitrogen (Fas-L [catalog 12-9919-42]; and Miltenyi Biotec (PD-1 [catalog 130-117-384], isotype IgG2b-PE [catalog 130-092-215], NKG2A [catalog 130-113-563]).

Myers J.A., Schirm D., Bendzick L., Hopps R., Selleck C., Hinderlie P., Felices M, & Miller J.S. (2022). Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion. JCI Insight, 7(15), e150079.

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