Biotinylated gsl 1 isolectin b4

Manufactured by Vector Laboratories

Biotinylated GSL I–isolectin B4 is a lectin derived from the seeds of the Griffonia simplicifolia plant. It is conjugated with biotin, a small molecule that binds to streptavidin with high affinity. This product can be used as a tool in various biomedical research applications.

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Lab products found in correlation

Laminin, by Merck Group (1 mentions) α bungarotoxin, by Thermo Fisher Scientific (1 mentions) Collagen 1, by Cedarlane (1 mentions) Synaptophysin, by Abcam (1 mentions) Hsp47, by Abcam (1 mentions) Lamin a, by Abcam (1 mentions) Anti occludin, by Thermo Fisher Scientific (1 mentions) Anti zo 1, by Thermo Fisher Scientific (1 mentions) Anti f actin, by Abcam (1 mentions) Alexafluor and texas red avidin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Isolectin B4 (42 citations) Biotinylated isolectin B4 (27 citations) GSL I-isolectin B4 (6 citations) (GSL I; (4 citations)

4 protocols using biotinylated gsl 1 isolectin b4

Quantitative Endothelial Cell Detection

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Biotinylated lectin (biotinylated GSL I–isolectin B4; Vector Labs) was used to detect mouse endothelial cells. Immunohistochemical analyses were performed as previously described.^{(1994 (link))} An Histostain SP kit (AEC Mouse Kit; Zymed Laboratories, Inc) appropriate for the primary antibody was used in the labeled‐[strept] avidin‐biotin technique. Lectin‐positive cells were counted using the imaging software as described earlier, and the number of positive cells per mm² thrombus area was documented. All counts and measurements were performed in a blinded fashion.

Frey M.K., Alias S., Winter M.P., Redwan B., Stübiger G., Panzenboeck A., Alimohammadi A., Bonderman D., Jakowitsch J., Bergmeister H., Bochkov V., Preissner K.T, & Lang I.M. (2014). Splenectomy Is Modifying the Vascular Remodeling of Thrombosis. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 3(1), e000772.

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Immunofluorescence Analysis of Neuromuscular Junctions

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The following antibodies were used in this study. The source of each antibody is indicated. Mouse: eMHC (DSHB, #F1.652, 1:50); CD31 (BD Pharmingen, #553371, 1:100); GFP (Invitrogen, #A11122, 1:250); Luciferase (Sigma-Aldrich, #L0159, 1:200); Collagen I (Cedarlane Labs, #CL50151AP, 1:200); HSP47 (Abcam, #ab77609, 1:200); α-Bungarotoxin (Life Technologies, # B-1196, 1:500); Laminin (Millipore, #MAB1903, 1:750); Synaptophysin (Abcam, #ab32594, 1:300); Neurofilament (Life Technologies, #MA5-14981, 1:200). Human: Lamin-A (Abcam, #ab108595, 1:200); Integrin (AbD Serotec, # MCA699PE, 1:200). Secondary antibodies (AlexaFluor, Invitrogen,1:1,000). Biotinylated GSL I-IsolectinB4 (Vector Laboratories, #B-1205).

Quarta M., Cromie M., Chacon R., Blonigan J., Garcia V., Akimenko I., Hamer M., Paine P., Stok M., Shrager J.B, & Rando T.A. (2017). Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss. Nature Communications, 8, 15613.

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Quantifying Microvascular Density and Capillary Domains

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Endothelial-specific immunohistochemistry staining was performed with Biotinylated GSL I–isolectin B4 (Vector Laboratories, Inc.) on freshly cut sections. Whole slide images were analyzed for microvascular density using the Image-Scope 10.0 microvessel analysis algorithm. For capillary domain area studies, analyzed images of the tissue for microvessel density were transferred to Image J software (4). Capillary domain areas (CDA) to determine tissue cross-sectional area that is closer to a given capillary than to any other were defined by creating Voronoi polygons around each vessel using Voronoi tessellation. Capillary domain surface area was measured for each capillary centered polygon and exported to Microsoft excel 2010 (Microsoft) for further analysis (Supplemental Figure 2).

Javan H., Szuscik A.M., Li L., Schaaf C.L., Salama M.E, & Selzman C.H. (2014). Cardiomyocyte p65 NF-κB Is Necessary for Compensatory Adaptation to Pressure-Overload: Javan et al: NF-κB and Pressure-Overload. Circulation. Heart failure, 8(1), 109-118.

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Immunostaining of Tight Junction Proteins in ARPE Cells

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Hcy-treated ARPE cells were fixed in 4% paraformaldehyde for 10 min, washed with PBS, and blocked with Power Block BioGenex, Fremont, CA), Ca. # BS-1310-25 for one hour. Cells were then incubated for 3h at 37°C with anti-ZO1 (Life technology, Cat#: 40-2200402200, diluted at 1/200), anti-occludin (Invitrogen, Cat#: 331500, diluted at 1/200), anti-F-actin (Abcam, ab205, diluted at 1/200) or Biotinylated GSL I - isolectin B4 (Vector Laboratories, Burlingame, Ca), Cat#: B-1205, 7μl/ml). Thereafter, cells were washed 3 times with PBS containing 0.3% Triton-X, incubated with appropriate secondary antibodies (Alexafluor and Texas red avidin, Invitrogen, Eugene, Oregon), and coverslipped with Fluoroshield containing DAPI (Sigma-Aldrich Chemical Corp., St. Louis, MO) as a counter stain. Immunofluorescent signals were detected by confocal microscopy (LSM, Carl Zeiss).

Ibrahim A.S., Mander S., Hussein K.A., Elsherbiny N.M., Smith S.B., Al-Shabrawey M, & Tawfik A. (2016). Hyperhomocysteinemia disrupts retinal pigment epithelial structure and function with features of age-related macular degeneration. Oncotarget, 7(8), 8532-8545.

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