Model 2020 osmometer

Keeping the α-tocopherol content constant at 15% v/v in oil: surfactant mixture, the polysorbate-80 content in SE-AS emulsions was increased to obtain an SE-AS with a narrower PdI. The goal of this experiment was to obtain an SE-AS which maintains size and PdI post-filtration. The emulsions were formulated similarly as previously described. Measurement of the pH was performed using an Orion 3-star pH meter from Thermo Scientific (Waltham, MA, USA) and osmolality was measured on an Advanced Instruments model 2020 osmometer (Norwood, MA, USA). Size, PdI and percent content of the oils were evaluated using previously described methods.

For each unit, pH measurements were made using a SevenMultiTM pH-meter with an InLabTM Micro Pro glass electrode (Mettler-Toledo, Viroflay, France). Measures were preceded and followed by instrument validation using standard buffer solution of pH 4 and pH 7 (HANNAH^® Instrument, Tannerries, France). Osmolality was measured for each solution using an osmometer Model 2020 Osmometer^® (Advanced instruments Inc., Radiometer, SAS, Neuilly Plaisance, France). Turbidity was measured using a 2100Q Portable Turbidimeter (Hach Lange, Marne La Vallée, France), by pooling the five samples per analysed experimental condition and assay time to obtain the necessary volume for the analysis. The results were expressed in Formazin Nephelometric Units (FNU).

For each unit, osmolality was measured using Model 2020 osmometer (Advanced instruments Inc., Radiometer, SAS, Neuilly Plaisance, France). pH measurements were made with a SevenMultiTM pH-meter with an InLabTM Micro Pro glass electrode (Mettler-Toledo, Viroflay, France).
Turbidity was measured using a 2100Q Portable Turbidimeter (Hach Lange, Marne La Vallée, France), by the pooling of four samples per analysed experimental condition and assay time to obtain the necessary volume for the analysis. The results were expressed in Formazin Nephelometric Units (FNU).