Anti phospho stat3 tyr705

TCDD and DIM were purchased from Cambridge Isotope (Tewksbury, MA, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively. Human TGF-β and murine Flt3L and IL-2 were from eBioscience (San Diego, CA, USA). Mouse GM-CSF was from BioLegend (San Diego, CA, USA). Antibodies used in the present study included: anti-STAT3 and anti-phospho-STAT5 (Tyr 694), and anti-phospho-IκBα (Ser 32/36) from Santa Cruz Biotechnology (USA); anti-phospho-c-Src (Tyr416) and anti-STAT5 from Cell Signaling Technology (Danvers, MA, USA); anti-phospho-STAT3 (Tyr705) from BD Biosciences (Franklin Lakes, NJ, USA); FITC anti-mouse CD11c, Alexa Fluor 647 anti-mouse CD103, anti-CD3, anti-β actin, PE anti-mouse CD4, Alexa Fluor 488 anti-mouse FoxP3 from BioLegend.

I3S, I3C, and DNFB were purchased from Sigma-Aldrich (St. Louis, MO, USA); murine Flt3L from eBioscience (San Diego, CA, USA). Antibodies used in the present study are: anti-signal transducer and activator of transcription 3 (STAT3) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-phospho-c-Src (Tyr416) from Cell Signaling Technology (Danvers, MA, USA); anti-phospho-STAT3 (Tyr705) from BD Biosciences (Franklin Lakes, NJ, USA); FITC anti-mouse CD11c, Alexa Fluor 647 anti-mouse B220, anti-ERK, and anti-phospho-ERK (Thr202/Tyr204) from Biolegend (San Diego, CA, USA). CpG oligodeoxyribonucleotides (CpG 2216: sequences are shown 5′-3′: small letters, phosphoorothioate linkage; capital letters, phosphodiester linkage 3′ of the base; bold, CpG-dinucleotides; ggGGGACGATCGTCgggggG) were manufactured by and purchased from Bioneer (Daejeon, Korea).