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Rotenone and antimycin

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Rotenone and antimycin are laboratory reagents used in biochemical research. Rotenone is a pesticide that inhibits the electron transport chain in mitochondria, while antimycin is an antibiotic that also disrupts the electron transport chain. These compounds are commonly used as tools to study cellular respiration and energy production processes.

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Lab products found in correlation

Oligomycin, by Agilent Technologies (2 mentions) Plastic culture plates, by Corning (1 mentions) Y 27632, by Fujifilm (1 mentions) Sb203580 sb2, by Cayman Chemical (1 mentions) Carbonyl cyanide 4 trifluoromethoxy phenylhydrazone fccp, by Agilent Technologies (1 mentions) Uk5099, by Agilent Technologies (1 mentions) Etomoxir, by Agilent Technologies (1 mentions) Seahorse xfe24, by Agilent Technologies (1 mentions) Cell tak, by Corning (1 mentions)

Close spelling variants of this product

Rotenone and antimycin A (5 citations) Rotenone/antimycin (4 citations) Rotenone (81 citations) Antimycin (11 citations)

2 protocols using rotenone and antimycin

1

Promoting Cell Differentiation and Proliferation

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The rho-associated protein kinase inhibitor Y-27632 (Y) to promote the cell differentiation and epidermal growth factor (EGF) originally used to promote the cell proliferation5 (link),7 (link) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan), and a p38 MAP kinase inhibitor SB203580 (SB2) from Cayman Chemical (Ann Arbor, MI) was used to block the cell senescence. SB431543 (SB4), originally thought to block the negative effect of transforming growth factor-β on cultures,5 (link),7 (link) Dulbecco's modified Eagle's medium–high glucose and fetal bovine serum were obtained from Gibco Industries Inc. (Langley, OK), and plastic culture plates were obtained from Corning (Corning, Inc., Corning, NY). Oligomycin, carbonyl cyanide 4-(trifluoromethoxy) phenyl-hydrazone (FCCP), rotenone and antimycin, UK5099, Bis-2-(5-phenylacetamido-1.3.4-thiadiazol-2-yl) ethyl sulfide (BPTES), and etomoxir were purchased from Agilent Technologies.
Numa K., Ueno M., Fujita T., Ueda K., Hiramoto N., Mukai A., Tokuda Y., Nakano M., Sotozono C., Kinoshita S, & Hamuro J. (2020). Mitochondria as a Platform for Dictating the Cell Fate of Cultured Human Corneal Endothelial Cells. Investigative Ophthalmology & Visual Science, 61(14), 10.
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2

Metabolic Profiling of Bone Marrow-Derived Cells

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Metabolic effect of stimulation of BMDCs was measured using two assays. For measurement of oxidative phosphorylation, the oxygen consumption rate (OCR) was analyzed by performing an overnight stimulation of BMDCs with either 12.5 μg/mL of PBC micelles, 1 μg/mL of LPS, or untreated control, was carried out in 5 mL polypropylene tubes. 2.5 × 105 stimulated BMDCs were seeded into Cell-Tak (Corning, Corning NY) coated Seahorse plates and a mitochondrial stress test (MST) was conducted according to manufacturer’s specifications using kit concentrations of 1 μM oligomycin, 2 μM FCCP, and 0.5 μM rotenone and antimycin (Agilent, Santa Clara, CA).
For measurement of acute metabolic responses and glycolysis (demonstrated by extracellular acidification rate, ECAR) upon stimulation, untreated BMDCs were seeded into Cell-Tak (Corning, Corning NY) coated seahorse plates at a density of 2.5 × 105 untreated BMDCs per well. Baseline metabolic activity readings were measured, and wells were stimulated with 12.5 μg/mL of micelle, 1 μg/mL of LPS, or medium control, and metabolic measurements are taken over the course of the assay. All metabolic phenotyping was conducted on a Seahorse XFe24 (Agilent, Santa Clara, CA).
Senapati S., Darling R.J., Loh D., Schneider I.C., Wannemuehler M.J., Narasimhan B, & Mallapragada S.K. (2019). Pentablock Copolymer Micelle Nanoadjuvants Enhance Cytosolic Delivery of Antigen and Improve Vaccine Efficacy while Inducing Low Inflammation. ACS biomaterials science & engineering, 5(3), 1332-1342.
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