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2 protocols using rabbit anti mouse alexa594

1

Multicolor Immunofluorescence Labeling

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Rabbit-anti-mouse-Alexa594 (Invitrogen #A11062); goat-anti-mouse-Alexa488 (Invitrogen #A11001); rabbit-anti-mouse-Alexa633 (Invitrogen #A21063); chicken-anti-rabbit-Alexa594 (Invitrogen #A21442); goat-anti-rabbit-Alexa488 (Invitrogen #A11008); goat-anti-rabbit-Alexa633 (Invitrogen #A21070).
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2

Modulation of AMPK Signaling in ZIKV Infection

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The antibodies used in the study were: anti-phospho (Thr172)-AMPKα, anti-phospho acetyl-CoA carboxylase (Ser79) (ACC), anti-AMPKα, anti-HK 2 (Cell Signaling Technology, Beverly, MA), anti-flavivirus 4G2 IgG antibody (EMD Millipore) anti-ZIKV NS3 (Genetax), anti-GLUT1 (Santa Cruz Biotechnologies), at dilutions of 1:1000, and anti-β-actin (Sigma Aldrich) at a dilution of 1:5000. The secondary antibodies used were goat anti-rabbit/mice-IgG-HRP (Bio-Rad, Hercules, CA), rabbit anti-mouse Alexa 594 (Invitrogen). The drugs used in the study were AICAR (1 mM) (Toronto Research Chemicals, Inc. (ON, Canada), Compound C (10 μM) (Toronto Research Chemicals, Inc. (ON, Canada), GSK621 (10 μM) (Sigma-Aldrich), 2-DG (1 mM) (Sigma-Aldrich) and Metformin (20 mM) (Ranbaxy Laboratories (Princeton, NJ). For AMPK inhibition and activation studies, cells were pre-treated with respective drugs for an hour prior to virus challenge, removed during adsorption and then added again in fresh media and kept for the entire duration of the experiment.
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