Rabbit anti α synuclein

Immunoblots were carried out 24 h after transfection as previously described (Dinter et al., 2016 (link)) using NP40 lysis buffer containing protease inhibitors (Pierce, Thermo Fisher Scientific) and the following primary antibodies: rabbit anti- $\alpha$ -synuclein (1:500, No. 2642, Cell Signalling Technology, Danvers, USA), mouse anti-beta-tubulin (1:1000, E7, Developmental Studies Hybridoma Bank, Iowa, USA). Secondary antibodies were anti-mouse IgG (NXA931) and anti-rabbit IgG (NA934V) from GE Healthcare Life Sciences (1:10000). These antibodies produce several nonspecific bands that are also visible in cells not expressing $\alpha$ -synuclein. Among the bands around 20 kDa observed with the $\alpha$ -synuclein antibody, only the upper band is considered specific and was used for quantification (see Dinter et al., 2016 (link) for details).

Dulbecco's Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS) and other cell culture reagents, goat anti-mouse- AlexaFluor594 (H + L) antibody, goat anti-rabbit-AlexaFluor488 (H + L) antibody and rabbit IgG isotype control antibody were obtained from Thermo Fisher Scientific (Invitrogen). si-m-PYCARD/ASC RNAs were obtained from Guangzhou RiboBio Co., Ltd. Mouse anti-NLRP3 (Cat# AG-20B-0014), mouse anti-caspase-1 (Cat# AG-20B-0042) and rabbit anti-ASC (Cat# AG-25B-0006) were purchased from AdipoGen Life Sciences. Rabbit anti-α-synuclein (Cat# 4179), rabbit anti-Phospho-α-synuclein (Cat# 23706), rabbit anti-phospho-NF-κB p65 (Cat# 3033) and mouse anti-IL-1β (Cat# 12242) were purchased form Cell Signaling Technology. rabbit anti-ASC (Cat# YT0365) was purchased from ImmunoWay Biotechnology. Mouse anti-Phospho-α-synuclein (Cat# pSyn #64) and rabbit anti-IBA1 (Cat# 019-19741) were purchased from FUJIFILM Wako Pure Chemical Corporation. Mouse anti-α-synuclein (Cat# sc-12767) and mouse anti-GSDMD (Cat# sc-393581) was purchased from Santa Cruz Biotechnology. Rabbit anti-NF-κB p65 (Cat# ab16502), goat anti-IBA1 (Cat# ab5076) and rabbit anti-GSDMD (Cat# ab219800) were purchased from Abcam. Rabbit anti-IL18 (Cat# A1115) was purchased from ABclonal. Rabbit anti-tyrosine hydroxylase (Cat# AB152) was purchased from Merck Millipore.