Human c5a

Manufactured by Complement Technology

Human C5a is a laboratory reagent used for scientific research purposes. It functions as a component of the complement system, a part of the immune response. The detailed specifications and intended use of this product are not available for presentation in an unbiased and factual manner.

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Lab products found in correlation

Attune nxt, by Thermo Fisher Scientific (2 mentions) Iscove s modified dulbecco s medium imdm, by Thermo Fisher Scientific (1 mentions) Microcentrifuge tube, by Eppendorf (1 mentions) Sb202190, by InvivoGen (1 mentions) C di amp, by InvivoGen (1 mentions) Bx795, by InvivoGen (1 mentions) U0126, by InvivoGen (1 mentions) Wortmannin, by InvivoGen (1 mentions)

3 protocols using human c5a

Neutrophil Phagocytosis Assay

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Purified human neutrophils, suspended in Iscoves Modified Dulbecco’s Medium (IMDM; Life Technologies) with 1% autologous serum at a concentration of 5 x 10⁶/mL were incubated in microcentrifuge tubes (Eppendorf) with purified human C5a (Complement Technology) or vehicle control. pHrodo-conjugated S. aureus or E. coli bioparticles (Life Technologies) were opsonized in 50% autologous serum for 30 minutes before be being added to the suspended cells. Analysis was by flow cytometry (Attune NxT, Thermo Fisher Scientific)

Wood A.J., Vassallo A.M., Ruchaud-Sparagano M.H., Scott J., Zinnato C., Gonzalez-Tejedo C., Kishore K., D’Santos C.S., Simpson A.J., Menon D.K., Summers C., Chilvers E.R., Okkenhaug K, & Morris A.C. (2020). C5a impairs phagosomal maturation in the neutrophil through phosphoproteomic remodeling. JCI Insight, 5(15), e137029.

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Listeria Monocytogenes Infection Assay

Cited 1 time

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Human C5a and C3a were purchased from Complement Technology. The C5aR agonist (C5aA) (RAARISLGPRSIKAFTE) (29 (link)) and C3aR agonist (C3aA) (WWTRRWRGDKLGLAR) (30 (link)) were synthesized by GenScript with greater than 95% purity. c-di-AMP, BX795, Wortmannin, U0126, and SB202190 were purchased from Invivogen. LPS from E. coli was purchased from List Labs, and CGI-1746 was purchased from ApexBio. Listeria monocytogenes ATCC strain 13932 (serotype 4b) (MicroBioLogics, Inc.) was used for the infection studies. Bacteria were cultured in Bacto brain heart infusion broth at 37°C to mid-logarithmic phase, harvested by centrifugation, washed once in sterile PBS, and resuspended in sterile PBS.

Mueller-Ortiz S.L., Calame D.G., Shenoi N., Li Y.D, & Wetsel R.A. (2017). The Complement Anaphylatoxins, C5a and C3a, Suppress Interferon-Beta Production in Response to Listeria monocytogenes by Inhibition of the Cyclic Dinucleotide-Activated Cytosolic Surveillance Pathway. Journal of immunology (Baltimore, Md. : 1950), 198(8), 3237-3244.

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Single-Cell Stimulation with Native C5a

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Native (not recombinant) human C5a (Complement Technology Inc.) was diluted in serum-free buffer to the desired concentration and preloaded into the pipette facing the cells. To minimize contamination of the experiment chamber, we kept the C5a pipette under a slight suction (negative) pressure until the start of each single-cell test. Once the cell-holding pipette and the C5a pipette had been positioned at the desired distance from one another, the pressure in the C5a pipette was reversed to the desired positive value, causing its solution to be expelled in a jet directed toward the cell.

Francis E.A, & Heinrich V. (2018). Extension of chemotactic pseudopods by nonadherent human neutrophils does not require or cause calcium bursts. Science signaling, 11(521), eaal4289.

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