Human lung adenocarcinoma epithelial cells (A549) and rat alveolar type II cells (RLE-6TN) were purchased from the American Type Culture Collection. A549 cells are a suitable cell model that display numerous properties in common with human alveolar epithelial cells; therefore, they are often used to research the mechanism of pulmonary fibrosis (34 (link),35 (link)). A549 cells were cultured in DMEM (high glucose) supplemented with 10% heat-inactivated FBS, while RLE-6TN cells were cultured in F-12 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% heat-inactivated FBS. A549 and RLE-6TN cells were maintained at 37°C in a humidified incubator containing 5% CO2. Cells were treated with PQ (800 µmol/l for A549 cells and 160 µmol/l for RLE-6TN cells; dissolved in PBS) for 24 h. The morphological alterations of cells were observed using phase contrast microscopy (Thermo Fisher Scientific, Inc.).
Rle 6tn
Manufactured by Thermo Fisher Scientific
Sourced in United States
The RLE-6TN is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform a specific function within the laboratory setting, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Additional information about the intended use or capabilities of this product is not available.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
F12 medium, by Thermo Fisher Scientific (2 mentions)
Rle 6tn, by American Type Culture Collection (2 mentions)
Hydrocortisone, by Thermo Fisher Scientific (1 mentions)
Transferrin, by Thermo Fisher Scientific (1 mentions)
Sodium selenite, by Thermo Fisher Scientific (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Mle 12, by Thermo Fisher Scientific (1 mentions)
Insulin like growth factor, by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Phase contrast microscopy, by Thermo Fisher Scientific (1 mentions)
3 protocols using rle 6tn
1
Paraquat-induced Lung Cell Damage
2
Culturing Human and Rodent Lung Epithelial Cells
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Human alveolar epithelial cell line (A549) was obtained from ATCC (CCL-185). The cells were cultured in DMEM with 10% fetal bovine serum (GIBCO Laboratories, Grand Island, NY), penicillin [100 U/ml] and streptomycin [100 mg/ml] at 37°C in a gas mixture of 5% CO2 / 95% air in 25 cm2 culture flasks (T-25; Corning, Costar). After reaching early confluence the cells were trypsinized and plated for experiments.
Lung epithelial cell lines from mice (MLE-12; CRL-2110) and rat (RLE-6TN; CRL-2300) were purchased from ATCC (Manassas, VA). MLE-12 cells were cultured in DMEM: F-12 medium with insulin (5 ng/ml), transferrin (0.01 mg/ml), sodium selenite (30 nM, hydrocortisone (10 nM), B-estradiol (10 nM), HEPES 10 nM, glutamine and 10% FBS (GIBCO Laboratories, Grand Island, NY). RLE-6TN cells were grown in Ham's F12 medium supplemented with bovine pituitary extract (0.01 mg/ml), insulin (5 ng/ml), insulin-like growth factor (2.5 ng/ml), transferrin (1.25 μg/ml), EGF (2.5 ng/ml), and 10% SFB (GIBCO Laboratories, Grand Island, NY).
Lung epithelial cell lines from mice (MLE-12; CRL-2110) and rat (RLE-6TN; CRL-2300) were purchased from ATCC (Manassas, VA). MLE-12 cells were cultured in DMEM: F-12 medium with insulin (5 ng/ml), transferrin (0.01 mg/ml), sodium selenite (30 nM, hydrocortisone (10 nM), B-estradiol (10 nM), HEPES 10 nM, glutamine and 10% FBS (GIBCO Laboratories, Grand Island, NY). RLE-6TN cells were grown in Ham's F12 medium supplemented with bovine pituitary extract (0.01 mg/ml), insulin (5 ng/ml), insulin-like growth factor (2.5 ng/ml), transferrin (1.25 μg/ml), EGF (2.5 ng/ml), and 10% SFB (GIBCO Laboratories, Grand Island, NY).
3
Modeling Alveolar Epithelial Cell Senescence
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Rat type II alveolar epithelial cell line (RLE-6TN) was purchased from the American Type Culture Collection (ATCC; Manassas, USA), and rat embryonic fibroblast CCC-REPF-1 was obtained from the Chinese Academy of Medical Sciences (Beijing, China).
All cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) under 5% CO 2 atmosphere at 37 °C.
To build the model of alveolar epithelial cell senescence, RLE-6TN cells were stimulated with different concentrations of BLM (0, 2.5, and 5 μM) for 72 h as previously described (Zhang et al. 2020) . For treatment, RLE-6TN cells were pre-treated with quercetin (5, 25, and 50 μM) for 1 h prior to BLM stimulation.
In addition, to simulate alveolar epithelial cell senescence-secreted SASP and observe its effects on lung fibroblast cells, RLE-6TN-derived conditional medium (CM) was harvested to further culture CCC-REPF-1 cells for 48 h.
All cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) under 5% CO 2 atmosphere at 37 °C.
To build the model of alveolar epithelial cell senescence, RLE-6TN cells were stimulated with different concentrations of BLM (0, 2.5, and 5 μM) for 72 h as previously described (Zhang et al. 2020) . For treatment, RLE-6TN cells were pre-treated with quercetin (5, 25, and 50 μM) for 1 h prior to BLM stimulation.
In addition, to simulate alveolar epithelial cell senescence-secreted SASP and observe its effects on lung fibroblast cells, RLE-6TN-derived conditional medium (CM) was harvested to further culture CCC-REPF-1 cells for 48 h.
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