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2 protocols using ecl fitc

1

Integrin-Mediated Cell Adhesion Assay

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Antibody against B4GALT1 was obtained from Abnova (Cat#PAB20512), while that against integrin β1 (CD29) was purchased from BD Transduction Laboratories (Cat#610468). Further, antibody against integrin α6 was obtained from Cell Signaling Technology Inc. (Cat#3750), while that against GAPDH was purchased from Meridian Life Science. (Cat#H86504M). Functional blocking antibodies for integrin β1 (P4C10) were obtained from Merck Millipore (Cat#MAB1987Z). Further, functional blocking antibodies for integrin α6 (CD49f) were purchased from Invitrogen (Cat#12-0495-82), and human collagen I, human collagen IV, human fibronectin, laminin, or bovine serum albumin (BSA) were purchased from Sigma Aldrich (St Louis, MO, USA). GSL-II-FITC, LEL-FITC, RCA-I-FITC, ECL-FITC, MAL-II-FITC, and PNA-FITC were purchased from Vector Laboratories (Burlingame, CA, USA).
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2

Integrin-Mediated Cell Adhesion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody against B4GALT1 was obtained from Abnova (Cat#PAB20512), while that against integrin β1 (CD29) was purchased from BD Transduction Laboratories (Cat#610468). Further, antibody against integrin α6 was obtained from Cell Signaling Technology Inc. (Cat#3750), while that against GAPDH was purchased from Meridian Life Science. (Cat#H86504M). Functional blocking antibodies for integrin β1 (P4C10) were obtained from Merck Millipore (Cat#MAB1987Z). Further, functional blocking antibodies for integrin α6 (CD49f) were purchased from Invitrogen (Cat#12-0495-82), and human collagen I, human collagen IV, human fibronectin, laminin, or bovine serum albumin (BSA) were purchased from Sigma Aldrich (St Louis, MO, USA). GSL-II-FITC, LEL-FITC, RCA-I-FITC, ECL-FITC, MAL-II-FITC, and PNA-FITC were purchased from Vector Laboratories (Burlingame, CA, USA).
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