Hacat human keratinocyte cell line

Manufactured by American Type Culture Collection

Sourced in United States

The HaCaT human keratinocyte cell line is a well-established in vitro model derived from normal human keratinocytes. It is a spontaneously immortalized cell line that retains many characteristics of normal keratinocytes. The HaCaT cell line is commonly used for research purposes to study skin biology, disease mechanisms, and the efficacy of various treatments.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) B16f10 murine melanoma, by Korean Cell Line Bank (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hela cervical cancer cell line, by American Type Culture Collection (1 mentions) Trypsin edta, by GE Healthcare (1 mentions) Rpmi 1640, by GE Healthcare (1 mentions) Penicillin and streptomycin solution, by GE Healthcare (1 mentions) Sh sy5y human neuroblastoma cell line, by American Type Culture Collection (1 mentions) Hct116 colorectal carcinoma cell line, by American Type Culture Collection (1 mentions)

Spelling variants of this product

HaCaT (257 citations) HaCaT cells (110 citations) HaCaT cell line (50 citations) HaCaT keratinocytes (12 citations) Human HaCaT keratinocytes (8 citations) HaCat line (2 citations)

6 protocols using hacat human keratinocyte cell line

In Vitro and In Vivo Antioxidant Evaluation

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SZR I and SZR II
were from our laboratory.^{16 (link)} Ginsenoside
Rb1, jujuboside A, jujuboside B, and spinosin standards were purchased
from Pufei De Biotech Co. (Chengdu, China). Macroporous resin (D101)
was obtained from Dongju Experimental Instrument Co. (Guangzhou, China).
Human MMP-1 ELISA was purchased from Neobioscience (Guangzhou, China).
Reactive oxygen species (ROS) was purchased from Meilunbio (Dalian,
China). Catalase (CAT), superoxide dismutase (SOD), and glutathione
peroxidase (GSH-PX) were obtained from Nanjing Jiancheng Bioengineering
Institute. HaCaT human keratinocyte cell line was purchased from ATCC
cell bank. C. elegans Bristol N2 and Escherichia coli OP50 were purchased from American
Caenorhabditis Genetics Center.

Hao K.X., Xie H., Jiang J.G., Wang D, & Zhu W. (2023). Semen Ziziphus jujube Saponins Protects HaCaT Cells against UV Damage and Alleviates the Aging of Caenorhabditis elegans. ACS Omega, 8(31), 28080-28089.

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Culturing Human Cell Lines

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HaCaT human keratinocyte cell line (ATCC, Manassas, VA, USA), CCD986Sk human fibroblast, and B16F10 murine melanoma cell lines (Korean Cell Line Bank, Seoul, Korea) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum and 1% streptomycin/penicillin at 37°C in a humidified incubator containing 5% CO2.

Lee J., Ji J, & Park S. (2018). Antiwrinkle and antimelanogenesis activity of the ethanol extracts of Lespedeza cuneata G. Don for development of the cosmeceutical ingredients. Food Science & Nutrition, 6(5), 1307-1316.

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Evaluating PAA and PAB Effects on Keratinocytes

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The HaCaT human keratinocyte cell line was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in DMEM supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and penicillin-streptomycin (100 U/mL; Gibco). Cells were grown at 37 °C in 100 mm dishes in a humidified atmosphere containing 5% CO₂. The cells were passaged three times a week, and the number of passages was within 10. Briefly, HaCaT cells were seeded in 100 mm plates (1.5 × 10⁶ cells/mL) and grown for 24 h. After treatment with various concentrations of PAA (100, 500, and 1000 μg/mL) and PAB (10, 30, and 60 μg/mL) with TNF-α and IFN-γ (10 ng/mL each) or without, the cells were incubated for 24 h. The cells were collected for real-time RT-PCR and western-blot analysis. Cell supernatants were collected and used for ELISA.

Ha Y., Lee W.H., Kim J.K., Jeon H.K., Lee J, & Kim Y.J. (2022). Polyopes affinis Suppressed IFN-γ- and TNF-α-Induced Inflammation in Human Keratinocytes via Down-Regulation of the NF-κB and STAT1 Pathways. Molecules, 27(6), 1836.

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Comparative Analysis of Cervical Cancer Cells

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The HeLa cervical cancer cell line [High-risk Human Papillomavirus (HPV)-18-positive] and the HaCaT human keratinocyte cell line (HPV-negative cell line) were purchased from the American Type Culture Collection (Manassas, VA, USA). HPVs encode E6 and E7 oncoproteins, which promote cervical cancer. Therefore, we used HaCaT cells as control cells to suggest anti-cancer effects of metformin in cervical cancer cell line (HeLa cells). HeLa and HaCaT cells were maintained in Dulbecco’s modified Eagle’s medium and minimum essential media in 5% CO₂ at 37°C. All media were supplemented with 10% foetal bovine serum (Invitrogen, Carlsbad, CA, USA), 100 μg/mL streptomycin, and 100 units/mL penicillin (Invitrogen).

Kim M.Y., Kim Y.S., Kim M., Choi M.Y., Roh G.S., Lee D.H., Kim H.J., Kang S.S., Cho G.J., Shin J.K, & Choi W.S. (2019). Metformin inhibits cervical cancer cell proliferation via decreased AMPK O-GlcNAcylation. Animal Cells and Systems, 23(4), 302-309.

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Probiotic Potential Evaluation in Human Cell Lines

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For the cell experiments, Lact. brevis D70 (100% identity; accession no. LC062897.1), Lact. pentosus S16 (100% identity; accession no. NR_029133.1), and Lact. fermentum MF10 (100% identity; accession no. NR_104927.1) were selected based on their probiotic potential.
The human HT-29 epithelial cell line was obtained from the Korea Cell Line Bank (KCLB, Seoul, South Korea). Cells were grown in maintenance medium supplemented with Roswell Park Memorial Institute (RPMI) 1640 (GE Healthcare, Chicago, IL, USA), 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco, Dublin, Ireland), and 1% (v/v) antibiotic solution (penicillin and streptomycin solution, 100 U mL - 1 ) (GE Healthcare).
The human HaCaT keratinocyte cell line was obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA). Cells were grown in maintenance medium supplemented with Dulbecco's modi ed Eagle's medium (DMEM) (GE Healthcare), 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco), and 1% (v/v) antibiotic solution (penicillin and streptomycin solution, 100 U mL - 1 ) (GE Healthcare). OD 517(control)
Cells were incubated at 37°C and 5% CO 2 in a humidi ed atmosphere, and the culture medium was replaced with fresh RPMI or DMEM maintenance medium every 2 d. Cells were transferred at 60% con uence using 1 mL of trypsin-EDTA (GE Healthcare).

Shin M.J., Lee C.S, & Kim S.H. (2023). Screening for Lactic Acid Bacterial Strains as Probiotics Exhibiting Anti-inflammatory and Antioxidative Characteristic Via Immune Modulation in HaCaT Cell. Probiotics and antimicrobial proteins, 15(6).

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Cell Culture Conditions for Cancer Cell Lines

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Human HaCaT keratinocyte cell line, human SH-SY5Y neuroblastoma cell line, and HCT 116 colorectal carcinoma cell line were purchased from ATCC (American Type Culture Collection). Hepatoblastoma HepG2 cell line was from ICLC (Interlab Cell Line Collection) at Istituto Nazionale per la Ricerca sul Cancro, Genoa (Italy). The cells were grown in DMEM high glucose medium supplemented with 10% Fetal Bovine Serum, 50.0 U/mL penicillin, and 100.0 μg/mL streptomycin, at 37 °C in a humidified atmosphere containing 5% CO₂.

Brahmi-Chendouh N., Piccolella S., Crescente G., Pacifico F., Boulekbache L., Hamri-Zeghichi S., Akkal S., Madani K, & Pacifico S. (2019). A nutraceutical extract from Inula viscosa leaves: UHPLC-HR-MS/MS based polyphenol profile, and antioxidant and cytotoxic activities. Journal of Food and Drug Analysis, 27(3), 692-702.

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