Glutathione cross linked to 4 agarose

Mouse monoclonal anti-Rac1 antibody was purchased from Cytoskeleton Inc. (Denver, CO, USA). Rabbit anti-GFP antibody was from Clontech (Mountain View, CA, USA). Mouse monoclonal anti-pRac1S71, anti-14-3-3s, 14-3-3η, -γ, -σ, and -θ, and anti-GST antibodies were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit monoclonal anti-α-tubulin antibody was from Abcam (Abcam Inc, Toronto, ON, Canada). FITC- and TRITC-conjugated donkey anti-mouse and anti-rabbit antibodies were from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA). Glutathione cross-linked to 4% agarose, goat anti-mouse IgG conjugated with agarose, and protein A conjugated with agarose were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mammalian Protein Extraction Reagent (M-Per) was purchased from Thermo Fisher Scientific Inc. (Rockford, IL USA). Unless otherwise specified, all chemicals were purchased from Sigma-Aldrich.

Mouse monoclonal (anti-phosphor ERK, p-ERK) and rabbit polyclonal (anti-ERK, anti-RhoA, anti-lamin A, anti-ROCK1, anti-mDia, anti-α-tubulin, anti-E-cadherin) and goat polyclonal anti-p-MYPT1(Thr 853) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Mouse monoclonal anti-threonine, anti-serine antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Rabbit anti-GFP antibody, was from Clonetech (Mountain View, CA). Purified His tagged RhoA protein was from Cytoskeleton Inc. (Denver, CO). Purified active ERK1 was purchased from SignalChem (Richmond, BC, Canada). U0126, Y-27632, Glutathione cross-linked to 4% agarose, goat anti-mouse IgG conjugated with agarose, protein A conjugated with agarose, and Amino Amido Black staining solution were purchased from Sigma-Aldrich (St. Louis, MO). Unless otherwise specified, all chemicals were purchased from Sigma-Aldrich.