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Acf enzymatic dissociation inhibition solution

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ACF Enzymatic Dissociation/Inhibition Solution is a laboratory reagent used to dissociate and inhibit enzymatic activity in cell samples. It is designed for use in cell culture applications that require the separation of cells while preserving their viability and functionality.

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Lab products found in correlation

Mesencult adipogenic differentiation kit, by STEMCELL (2 mentions) Mesencult acf chondrogenic differentiation medium, by STEMCELL (2 mentions) Mesencult acf basal medium, by STEMCELL (2 mentions) Mesencult osteogenic differentiation medium, by STEMCELL (2 mentions)

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ACF Enzymatic Dissociation Solution

2 protocols using acf enzymatic dissociation inhibition solution

1

Multilineage Differentiation of Mesenchymal Stem Cells

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Adipocytes, osteoblasts, and chondrocytes were generated from MSCs. For adipocyte differentiation, MSCs between passages 3 – 4 were plated on 6-well plates with MesenCult-ACF Basal Medium (StemCell Technologies, Cat. # 05449) at 4 – 5 3 105 cells per well. Differentiation was performed using the MesenCult Adipogenic Differentiation Kit (StemCell Technologies, Cat. # 05412) according to the manufacturer’s protocol. Media changes were done every 3 – 4 d until 13 d. For osteogenic lineage differentiation, MSCs between passages 3 – 4 were plated on a 6-well plate with MesenCult-ACF Basal Medium (StemCell Technologies) at 3 – 4 × 104 cells per well. Differentiation was performed using MesenCult Osteogenic Differentiation medium (StemCell Technologies, Cat. # 05465) according to the manufacturer’s protocol. Medium changes were done every 3 – 4 d until 13 d. For 3-D pellet chondrogenic differentiation, MSCs were first released from T25 flasks using ACF Enzymatic Dissociation/Inhibition Solution (StemCell Technologies, Cat. # 05426) and collected in polypropylene tubes at 2.5 – 3 × 106 cells per tube with MesenCult-ACF Chondrogenic Differentiation Medium (StemCell Technologies, Cat. # 05455) according to the manufacturer’s protocol. Medium changes were done every 3 – 4 days until 13 d.
Patananan A.N., Sercel A.J., Wu T.H., Ahsan F.M., Torres A J.r., Kennedy S.A., Vandiver A., Collier A.J., Mehrabi A., Van Lew J., Zakin L., Rodriguez N., Sixto M., Tadros W., Lazar A., Sieling P.A., Nguyen T.L., Dawson E.R., Braas D., Golovato J., Cisneros L., Vaske C., Plath K., Rabizadeh S., Niazi K.R., Chiou P.Y, & Teitell M.A. (2020). Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates. Cell reports, 33(13), 108562.
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2

Multilineage Differentiation of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adipocytes, osteoblasts, and chondrocytes were generated from MSCs. For adipocyte differentiation, MSCs between passages 3 – 4 were plated on 6-well plates with MesenCult-ACF Basal Medium (StemCell Technologies, Cat. # 05449) at 4 – 5 3 105 cells per well. Differentiation was performed using the MesenCult Adipogenic Differentiation Kit (StemCell Technologies, Cat. # 05412) according to the manufacturer’s protocol. Media changes were done every 3 – 4 d until 13 d. For osteogenic lineage differentiation, MSCs between passages 3 – 4 were plated on a 6-well plate with MesenCult-ACF Basal Medium (StemCell Technologies) at 3 – 4 × 104 cells per well. Differentiation was performed using MesenCult Osteogenic Differentiation medium (StemCell Technologies, Cat. # 05465) according to the manufacturer’s protocol. Medium changes were done every 3 – 4 d until 13 d. For 3-D pellet chondrogenic differentiation, MSCs were first released from T25 flasks using ACF Enzymatic Dissociation/Inhibition Solution (StemCell Technologies, Cat. # 05426) and collected in polypropylene tubes at 2.5 – 3 × 106 cells per tube with MesenCult-ACF Chondrogenic Differentiation Medium (StemCell Technologies, Cat. # 05455) according to the manufacturer’s protocol. Medium changes were done every 3 – 4 days until 13 d.
Patananan A.N., Sercel A.J., Wu T.H., Ahsan F.M., Torres A J.r., Kennedy S.A., Vandiver A., Collier A.J., Mehrabi A., Van Lew J., Zakin L., Rodriguez N., Sixto M., Tadros W., Lazar A., Sieling P.A., Nguyen T.L., Dawson E.R., Braas D., Golovato J., Cisneros L., Vaske C., Plath K., Rabizadeh S., Niazi K.R., Chiou P.Y, & Teitell M.A. (2020). Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates. Cell reports, 33(13), 108562.
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