Tissues were fixed in formalin and embedded in paraffin, and the sections were used for hematoxylin-eosin staining and IHC. For IHC, the sections were subjected to heat-mediated antigen retrieval in pH 6.0 citrate buffer after routine deparaffinization and rehydration. The following staining steps were conducted using a rabbit biotin-streptavidin horseradish peroxidase detection system (SP-9001, ZSGB-BIO, China). The staining images of tumor tissues were evaluated by two researchers, according to the staining intensity. The CD31 antibody (#77699, CST, USA) and endoglin antibody (ab221675, Abcam, UK) were diluted at 1:100. The ADAMTS2 antibody (PA5-78728, Invitrogen, USA) was diluted at 1:500. Finally, the sections were counterstained with hematoxylin.
Adamts2 antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
The ADAMTS2 antibody is a research tool used to detect and study the ADAMTS2 protein, which is involved in the processing of procollagen. It can be used in various laboratory techniques, such as Western blotting, immunohistochemistry, and ELISA, to identify and quantify the ADAMTS2 protein in biological samples.
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Lab products found in correlation
2 protocols using adamts2 antibody
1
Immunohistochemical Analysis of Tumor Tissues
2
Immunohistochemical Analysis of FTO
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IHC was performed as previous described27 and was employed to determine the expression of FTO protein in PC clinical specimens. The tissues were fixed with 4% paraformaldehyde, embedded in paraffin, and sectioned at 3 μm. Then use rabbit polyclonal anti‐THBS2 antibody (1:250, abclonal, A8561, China), ADAMTS2 Antibody (1:25, Invitrogen, PA5‐50539, USA), COL12A1 (1:50, abcam, ab121304, UK), and anti‐FTO antibody (1:250, abclonal, A3861, China) at 4°C overnight. After the secondary antibody was incubated, the sections were stained with the DAB reagent. The results were analyzed using Image‐Pro Plus 6.0 software.
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