Anti caveolin 1 n 20

Anti-caveolin-1 (N-20) and DUSP5 antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti- cleaved caspase-9, cleaved PARP, phospho-Akt, total Akt, phospho-BRCA1, phospho-DNAPK, total BRCA1, total DNAPK, phospho-JAK2, total JAK2, phospho-STAT3, total STAT3, PIAS3, Src (Y416), Src (Y527), total Src, SOCS2, phospho-JNK, total JNK, phospho-p38, total p38, phospho-ERK1/2, total ERK1/2, alpha tubulin, phospho-H2.AX, beta actin and GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA). Gemcitabine and 5-fluorouracil (5-FU) was purchased from Sigma-Aldrich (St. Louis, MO) and Santa Cruz Biotechnology (Dallas, TX) respectively. For in vitro studies, Gemcitabine and 5-FU were dissolved in DMSO. MIAPaCa-2, BxPC3, Panc-1, AsPC1, Capan-1, Capan-2, HPAFII and HEK-293 were obtained from and authenticated (via short tandem repeat profiling) by the American Type Culture Collection (Manassas, VA), and grown according to ATCC recommendations. HPDE cells were kindly provided by Dr Diane Simeone (University of Michigan). Cells used for this study were cryopreserved within 6 months of authentication. SW-48 and DLD-1 isogenic cell line pairs were obtained from Horizon Discovery (Cambridge, UK). Cells were passaged for no longer than 3 months and grown in a 37 °C incubator with 5% CO₂.