Sorbitan oleate

KDP was purchased from SM Empreendimentos Farmacêuticos Ltd.a. (São Paulo, Brazil); rosehip oil was purchased from Delaware (Porto Alegre, Brazil); sorbitan oleate was obtained from Croda (Campinas, Brazil); polysorbate 80 was obtained from Labsynth (Diadema, Brazil); tetrahydrofuran (THF) was purchased from Química Moderna (Barueri, Brazil); acetonitrile was obtained from J.T. Baker (State of Mexico, Mexico); methanol and tyrosinase (50 kU) were purchased from Sigma–Aldrich Brasil Ltd.a. (Cotia, Brazil); and 3T3-L1 cells were purchased from the Rio de Janeiro Cell Bank (BCRJ) (Rio de Janeiro, Brazil). All other chemicals or reagents were of analytical grade and used as received.

CBZ and sacha inchi oil (SIO) were provided by Tecnoquimicas S.A Pharmaceutical Company (Cali, Valle del Cauca, Colombia) and Nutresacha SA (Santander de Quilichao, Cauca, Colombia), respectively. Sorbitan oleate (Span^TM 80, hydrophilic-lipophilic balance (HLB) = 4.3) and Polysorbate 80 (Tween^TM 80, HLB = 15) were purchased from Croda (Snaith, UK). Methyl-paraben and propyl-paraben were acquired from Sigma-Aldrich (St. Louis, MO, USA). Water Type II (ultrapure water) was obtained via a Millipore Elix Essential purification system (Merck KGaA, Darmstadt, Germany), while the water employed for high-performance liquid chromatography (HPLC) was purified using a Sartorius Arium-Pro (Sartorius AG, Gotinga, Germany). All solvents were of analytical reagent grade.