The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (Thermo Fisher Scientific, 13-1900, 1 : 100 dilution), anti-Myosin-VIIa rabbit polyclonal antibody (Proteus BioSciences Inc., #25-6790, 1 : 200 dilution), anti-Histone H3 (phospho S28) rat polyclonal antibody (Abcam, #ab10543, 1 : 200 dilution), Alexa Fluor 546-conjugated goat anti-rat IgG (H + L) antibody (Thermo Fisher Scientific, #A11081, 1 : 1000 dilution) and Alexa Fluor 647-conjugated goat anti-rabbit IgG (H + L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1 : 1000 dilution). We used MMC (Nacalai Tesque, #20898-2).
Alexa fluor 647 conjugated goat anti rabbit igg h l antibody
Manufactured by Abcam
Alexa Fluor 647-conjugated goat anti-rabbit IgG (H + L) antibody is a secondary antibody used for detection and visualization in various immunoassays and imaging techniques. It is conjugated to the Alexa Fluor 647 fluorescent dye, which has excitation and emission maxima at 650 nm and 665 nm, respectively.
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2 protocols using alexa fluor 647 conjugated goat anti rabbit igg h l antibody
1
Immunofluorescence Staining for E-cadherin, Myosin-VIIa, and Histone H3
2
Immunofluorescence Staining of Cell Markers
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The following primary and secondary antibodies were used for immunofluorescence: anti-Ecadherin rat antibody (Cell Signaling Technology, #3195, 1:100 dilution), anti-Myosin-VIIa rabbit polyclonal antibody (Proteus BioSciences Inc., #25-6790, 1:200 dilution), anti-Histone H3 (phospho S28) rat polyclonal antibody (Abcam, #ab10543, 1:200 dilution), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (Thermo Fisher Scientific, #A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1:1000 dilution).
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