1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc

Manufactured by Energy Chemical

Sourced in China

1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) is a water-soluble carbodiimide compound commonly used as a coupling agent in organic synthesis. It facilitates the formation of amide bonds between carboxylic acids and primary amines.

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1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (5 citations)

4 protocols using 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc

Rapid Immunoassay for SARS-CoV-2 Detection

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AgNO₃, NaBH₄, Na₂SO₃, NaOH, KH₂PO₄, Na₂HPO₄, NaCl, H₂O₂, FeCl₃, ethylene glycol, sodium acetate, and KCl were of AR grade and used without additional purification. Trisodium citrate (TSC), L-ascorbic acid (L-AA), and polyvinylpyrrolidone (PVP, MW = 10,000) were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). N-hydroxysuccinimide (NHS), chloroauric acid (HAuCl₄), sucrose, MBA, and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Energy Chemical Co., Ltd (Shanghai, China). HS-(PEG)_n-COOH (MW = 2000) was purchased from ToYongBio Tech. Inc. (Shanghai, China). SARS-CoV-2 spike protein, mouse anti-N protein antibody, and rabbit anti-human IgG were purchased from Sino Biological Inc. (Beijing, China). Mouse anti-Staphylococcus aureus antibody and mouse anti-Salmonella typhimurium antibody and were purchased from Sigma-Aldrich (Saint Louis, MO, USA). SARS-CoV-2 N protein antibody was purchased from Abcam. Bovine serum albumin (BSA) was purchased from Labgic Technology Co., Ltd. (Beijing, China). Tween-20 was purchased from EKEAR Bio@Tech Co., Ltd. (Shanghai, China). Glass fiber conjugate pad, nitrocellulose membrane (NC membrane), PVC substrate, and absorbent pad were purchased from Joey-biotech Co., Ltd. (Shanghai, China). Serum samples were taken from the affiliated hospital of Qingdao University and stored at − 20 ℃ for use.

Li J., Liang P., Zhao T., Guo G., Zhu J., Wen C, & Zeng J. (2022). Colorimetric and Raman dual-mode lateral flow immunoassay detection of SARS-CoV-2 N protein antibody based on Ag nanoparticles with ultrathin Au shell assembled onto Fe3O4 nanoparticles. Analytical and Bioanalytical Chemistry, 415(4), 545-554.

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Lateral Flow Assay Reagent Synthesis

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Tri ethanolamine, cetyltrimethyl ammonium bromide (CTAB), sodium salicylate, tetraethyl silicate (TEOS), ethanol, concentrated hydrochloric acid (HCl), ammonia, β-(aminoethyl)-γ-aminopropyltrimethoxysilane (AEAPTMS), γ-aminopropyltriethoxysilane (APTES) citric acid (CA), sodium dihydrogen phosphate (MSP), absolute ethanol, N,N-Dimethylformamide (DMF), Tween-20, Polyvinyl Pyrrolidone (PVP), NaCl, and succinic anhydride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was purchased from Energy Chemical Co., Ltd. (Tangshan, China). Bovine serum albumin (BSA) was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). The procalcitonin antigen (PCT) and its matched labeled antibody and coated antibody were provided by Nanjing Okai Biotechnology Co., Ltd. (Nanjing, China). The AFP, CA199, and its matched labeled antibody and coated antibody were provided by the Jiangsu Provincial Center for Disease Control and Prevention. Goat anti-mouse immunoglobulin G (IgG), sample pad, nitrocellulose (NC) membrane, absorbent pad, and polyvinyl chloride (PVC) substrate were purchased from Shanghai Jieyi Biotechnology Co., Ltd. (Shanghai, China).

Hu J.X, & Ding S.N. (2023). In Situ Synthesis of Highly Fluorescent, Phosphorus-Doping Carbon-Dot-Functionalized, Dendritic Silica Nanoparticles Applied for Multi-Component Lateral Flow Immunoassay. Sensors (Basel, Switzerland), 24(1), 19.

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Functionalized Pyrazinophenanthroline for Cell Studies

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N-Hydroxysuccinimide (NHS, Energy Chemical, Shanghai, China), (2,3-f)-Pyrazino(1,10)phenanthroline-2,3-dicarboxy-lic acid (PPDA, Energy Chemical, Shanghai, China), Ethylenediamine (Energy Chemical), 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydro chloride (EDC, Energy Chemical, Shanghai, China), citric acid (Energy Chemical, Shanghai, China), HEPES (Energy Chemical, Shanghai, China), nitrate salts of metal ion and sodium salts of anion of analytic grade were used as received. HeLa cells (Human cervix carcinoma cell) were purchased from a cell bank in Shanghai (Shanghai, China).

Zhang L., Wang Z., Zhang J., Jia J., Zhao D, & Fan Y. (2018). Phenanthroline-Derivative Functionalized Carbon Dots for Highly Selective and Sensitive Detection of Cu2+ and S2− and Imaging inside Live Cells. Nanomaterials, 8(12), 1071.

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Osteoclastogenesis Inhibition by ALN

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Methotrexate was obtained from Aladdin (Shanghai, CN, USA). Acetone was obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, CN, USA). 2-Morpholinoethane-sulfonic acid (MES), N-hydroxysuccinimide (NHS), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were obtained from Energy Chemical Co. (Shanghai, CN, USA). ALN was purchased from Lullaby Pharmaceutical Chemical Co. (Wuhan, Hubei, CN, USA). All solutions were prepared using ultra-pure water from a Milli-Q water purification system (Millipore Corporation, Billerica, MA, USA). Dialysis bags were obtained from UC Union Carbide (Danbury, CT, USA). The purified solution was lyophilized by LYOQUEST-55 (Azibil Telstar Technologies S.L.U., Spain). The alpha modification of Eagle’s medium (α-MEM), penicillin/streptomycin, and fetal bovine serum (FBS) were purchased from Gibco-BRL (Gaithersburg, MD, USA). A cell counting kit (CCK-8) was obtained from Dojindo Molecular Technology (Kumamoto, Japan). Recombinant mouse M-CSF and mouse RANKL were obtained from R&D (Minneapolis, MN, USA). Rap1a antibody (sc-1482) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), while all other antibodies were obtained from Cell Signaling Technology (Cambridge, MA, USA). The tartrate-resistant acid phosphatase (TRAP) staining kit and all other reagents were purchased from Sigma Aldrich, unless otherwise stated.

Xie Z., Liu G., Tang P., Sun X., Chen S., Qin A., Zhu P., Zhang J, & Fan S. (2018). Bone-targeted methotrexate–alendronate conjugate inhibits osteoclastogenesis in vitro and prevents bone loss and inflammation of collagen-induced arthritis in vivo. Drug Delivery, 25(1), 187-197.

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