Luminaris color higreen reagents qpcr master mix

We used the ΔΔCt method to determine the expression of mRNA using real-time PCR: ΔΔCT = ΔCT test sample—ΔCT calibrator sample. The reaction was carried out using 48-well plates and Luminaris Color HiGreen reagents qPCR Master Mix (Thermo Fisher Scientific); 100 ng of cDNA was used for each reaction. The following genes were examined: casp3, casp9, cytc, aifm1, pcna, ki-67, and mcm2. The primers used for this procedure are presented in Table 2. rpl13a was used as the reference house-keeping gene [65 (link)]. The reaction conditions were set as specified by the manufacturer. Each sample was analyzed in duplicate. The procedure was conducted using a StepOnePlus™ Real-Time PCR System.

The ∆∆Ct method was used to determine the expression of mRNA using real-time PCR (Equation (2)):
The reaction was carried out using 48-well plates and the Luminaris Color HiGreen reagents qPCR Master Mix (Thermo Fisher Scientific); 100 ng of cDNA was used for each reaction. The following genes were examined: p21, p53, B-cell lymphoma 2 (Bcl-2), BAX and PCNA. The primers used for this procedure are presented in Table 1. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) was used as the reference housekeeping gene. The reaction conditions were set as specified by the manufacturer, and each sample was analysed in duplicate. The procedure was conducted using a StepOnePlus™ Real-Time PCR System. qPCR results were normalised to the control (Log2FC = 0). Relative expression was calculated using the GPDH gene and control group (0).

The ∆∆Ct method was used to determine the expression of mRNA, using real-time PCR:
The reaction was carried out using 48-well plates and the Luminaris Color HiGreen reagents qPCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA); 100 ng of cDNA were used for each reaction. The following genes were examined: caspase-3 and proliferating cell nuclear antigen (PCNA). The primers used for this procedure are presented in Table 1. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) was used as the reference house-keeping gene. The reaction conditions were set as specified by the manufacturer, and each sample was analyzed in duplicate. The procedure was conducted using a StepOnePlus™ Real-Time PCR System.